The role of aldehyde/alcohol dehydrogenase (AdhE) in ethanol production from glycerol by Klebsiella pneumoniae

Bioenergy/Biofuels/Biochemicals

Abstract

Transcriptome analysis of a K. pneumoniae GEM167 mutant strain derived by irradiation with gamma rays, which exhibited high-level production of ethanol from glycerol, showed that the mutant expressed AdhE at a high level. Ethanol production decreased significantly, from 8.8 to 0.5 g l−1, when an adhE-deficient derivative of that strain was grown on glycerol. Bacterial growth was also reduced under such conditions, showing that AdhE plays a critical role in maintenance of redox balance by catalyzing ethanol production. Overexpression of AdhE enhanced ethanol production, from pure or crude glycerol, to a maximal level of 31.9 g l−1 under fed-batch fermentation conditions; this is the highest level of ethanol production from glycerol reported to date.

Keywords

Klebsiella pneumoniae Glycerol Ethanol Aldehyde/alcohol dehydrogenase (AdhE) 

Supplementary material

10295_2012_1224_MOESM1_ESM.doc (327 kb)
Supplementary material 1 (DOC 327 kb)

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Copyright information

© Society for Industrial Microbiology and Biotechnology 2013

Authors and Affiliations

  1. 1.Applied Microbiology Research Center, Bio-Materials Research InstituteKorea Research Institute of Bioscience and Biotechnology (KRIBB)JeongeupKorea
  2. 2.Radiation Research Division for BiotechnologyKorea Atomic Energy Research InstituteJeonbukSouth Korea

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