Skip to main content
Log in

Denitrification of high nitrate wastewater in a cloth strip bioreactor with immobilized sludge

  • Environmental Microbiology
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Denitrification of synthetic high nitrate wastewater containing 40,000 ppm NO3 (9,032 ppm NO3-N) was achieved using immobilized activated sludge in a column reactor. Active anoxic sludge adsorbed onto Terry cloth was used in the denitrification of high nitrate wastewater. The operational stability of the immobilized sludge system was studied both in a batch reactor and in a continuous reactor. The immobilized sludge showed complete degradation of different concentrations of NO3-N (1,129, 1,693, 3,387, 6,774, and 9,032 ppm) in a batch process. The reactors were successfully run for 90 days without any loss in activity. The immobilized cell process has yielded promising results in attaining high denitrifying efficiency.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. APHA (2005) Standard methods for the examination of water and wastewater. American Public Health Association, Washington DC

    Google Scholar 

  2. Bickerstaff GF (1997) Methods in biotechnology. Humana, New Jersey

    Google Scholar 

  3. Biradar PM, Dhamole PB, Nair RR, Roy SB, Satpati SK, D’Souza SF, Lele SS, Pandit AB (2008) Long-term stability of biological denitrification process for high strength nitrate removal from wastewater of uranium industry. Environ Prog 27:365–372

    Article  CAS  Google Scholar 

  4. Boaventura RAR, Rodrigues AE (1997) Denitrification kinetics in a rotating disk biofilm reactor. Chem Eng J 65:227–235

    Article  CAS  Google Scholar 

  5. Delgenes JP, Rustrian E, Bernet N, Moleta R (1998) Combined biodegradation of carbon, nitrogen and phosphorous from wastewaters. J Mol Catal B Enzym 5:429–433

    Article  CAS  Google Scholar 

  6. Dhamole PB, Nair RR, D’Souza SF, Lele SS (2009) Simultaneous carbon and nitrate removal in an airlift bioreactor. Bioresour Technol 100:1082–1086

    Article  PubMed  CAS  Google Scholar 

  7. Dhamole PB, Nair RR, D’Souza SF, Lele SS (2008) Denitrification of highly alkaline nitrate waste. Appl Biochem Biotech 151:433–440

    Article  CAS  Google Scholar 

  8. Dhamole PB, Nair RR, D’Souza SF, Lele SS (2007) Denitrification of high strength nitrate waste. Bioresour Technol 98:247–252

    Article  PubMed  CAS  Google Scholar 

  9. D’Souza SF, Kubal BS (2002) A cloth strip bioreactor with immobilized glucoamylase. J Biochem Bioph Meth 51:151–159

    Article  Google Scholar 

  10. D’Souza SF, Melo JS (2001) Immobilization of bakers yeast on jute fabric through adhesion using polyethyleneimine: application in an annular reactor for the inversion of sucrose. Process Biochem 36:677–681

    Article  Google Scholar 

  11. D’Souza SF (1999) Immobilized enzymes in bioprocess. Curr Sci India 77:69–79

    Google Scholar 

  12. D’Souza SF, Kamath N (1988) Cloth bioreactor containing yeast cells immobilized on cotton cloth using polyethyleneimine. Appl Microbiol Biotechnol 29:136–140

    Article  Google Scholar 

  13. D’souza SF, Melo JS, Deshpande A, Nadkarni GB (1986) Immobilization of yeast cells by adhesion to glass surface using polyethylenimine. Biotechnol Lett 8:643–648

    Article  Google Scholar 

  14. Forman D (1991) Nitrate exposure and human cancer. In: Bogardi I, Kuzelka R (eds) Nitrate contamination: exposure, consequence and control. Springer, Berlin, pp 281–288

    Google Scholar 

  15. Glass C, Silverstein J (1999) Denitrification of high nitrate, high salinity wastewater. Water Res 33:223–229

    Article  CAS  Google Scholar 

  16. Gupta AB, Gupta SK (1999) Simultaneous carbon and nitrogen removal in a mixed culture aerobic RBC biofilm. Water Res 33:555–561

    Article  CAS  Google Scholar 

  17. Joshi S, Yamazaki H (1984) Film fermentor for ethanol production by yeast (Sachharomyces cerevisiae) immobilized on cotton cloth. Biotechnol Lett 6:797–802

    Article  CAS  Google Scholar 

  18. Kamath N, D’Souza SF (1992) Immobilization of ureolytic cells through flocullation and adhesion on cotton cloth using polyethylenimine. Enzyme Microb Tech 13:935–938

    Article  Google Scholar 

  19. Koenig A, Liu LH (2001) Kinetic model of autotrophic denitrification in sulphur packed bed reactors. Water Res 35:1969–1978

    Article  PubMed  CAS  Google Scholar 

  20. Lewis VP, Yang ST (1992) Continous propionic acid fermentation by immobilized Propionibacterium acidipropionici in a novel backed bed bioreactor. Biotechnol Bioeng 40:465–474

    Article  PubMed  CAS  Google Scholar 

  21. Marshall RC (1984) Microbial adhesion and aggregation. Springer, Berlin

    Book  Google Scholar 

  22. Melo JS, D’Souza SF (1999) Simultaneous filtration and immobilization of cells from a flowing suspension using a bioreactor containing polyethylenimine coated cotton threads: application in the continuous inversion of concentrated sucrose syrups. World J Microb Biot 15:17–21

    Article  Google Scholar 

  23. Munch EV, Lant P, Keller J (1996) Simultaneous nitrification and denitrification in bench scale sequencing batch reactors. Water Res 30:277–284

    Article  Google Scholar 

  24. Nair RR, Dhamole PB, Lele SS, D’Souza SF (2008) Biotreatment of high strength nitrate waste using immobilized preadapted sludge. Appl Biochem Biotech 151:193–200

    Article  CAS  Google Scholar 

  25. Nair RR, Dhamole PB, Lele SS, D’Souza SF (2007) Biological denitrification of high strength nitrate waste using preadapted denitrifying sludge. Chemosphere 67:1612–1617

    Article  PubMed  CAS  Google Scholar 

  26. USEPA (1993) Nitrogen control, EPA/625/R-93/010. Environmental Protection Agency, Washington DC

    Google Scholar 

  27. Zala SL, Ayyer J, Desai AJ (2004) Nitrate removal from the effluent of a fertilizer industry using a bioreactor packed with immobilized cells of Pseudomonas stutzeri and Comamonas testosterone. World J Microb Biot 20:661–665

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank the Department of Atomic Energy (India) for funding this work, and Rashtriya Chemicals and Fertilizers, Mumbai (India) for providing the sludge.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Rashmi R. Nair.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nair, R.R., D’Souza, S.F. Denitrification of high nitrate wastewater in a cloth strip bioreactor with immobilized sludge. J Ind Microbiol Biotechnol 39, 1637–1643 (2012). https://doi.org/10.1007/s10295-012-1175-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10295-012-1175-0

Keywords

Navigation