Abstract
We describe a latex wet coalescence method for gas-phase immobilization of microorganisms on paper which does not require drying for adhesion. This method reduces drying stresses to the microbes. It is applicable for microorganisms that do not tolerate desiccation stress during latex drying even in the presence of carbohydrates. Small surface area, 10–65 μm thick coatings were generated on chromatography paper strips and placed in the head-space of vertical sealed tubes containing liquid to hydrate the paper. These gas-phase microbial coatings hydrated by liquid in the paper pore space demonstrated absorption or evolution of H2, CO, CO2 or O2. The microbial products produced, ethanol and acetate, diffuse into the hydrated paper pores and accumulate in the liquid at the bottom of the tube. The paper provides hydration to the back side of the coating and also separates the biocatalyst from the products. Coating reactivity was demonstrated for Chlamydomonas reinhardtii CC124, which consumed CO2 and produced 10.2 ± 0.2 mmol O2 m−2 h−1, Rhodopseudomonas palustris CGA009, which consumed acetate and produced 0.47 ± 0.04 mmol H2 m−2 h−1, Clostridium ljungdahlii OTA1, which consumed 6 mmol CO m−2 h−1, and Synechococcus sp. PCC7002, which consumed CO2 and produced 5.00 ± 0.25 mmol O2 m−2 h−1. Coating thickness and microstructure were related to microbe size as determined by digital micrometry, profilometry, and confocal microscopy. The immobilization of different microorganisms in thin adhesive films in the gas phase demonstrates the utility of this method for evaluating genetically optimized microorganisms for gas absorption and gas evolution.
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Acknowledgments
Support for these studies was provided to J.L.G., M.S.C., A.M.G., and M.C.F. by North Carolina Biotechnology Center (NCBC) grant number 2008-MRG-1104. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views and policies of the NCBC. J.S.J. was supported by a NCSU/NIH Molecular Biotechnology Training Program Traineeship. O.I.B. was supported by the Office of Fossil Energy—National Energy Technology Laboratory through subcontract AC 70110 O from Savannah River Nuclear Solutions, LLC. The authors would like to thank Orlin Velev and members of his laboratory group for assistance with the confocal laser scanning microscopy. All authors have agreed to submit this manuscript to Journal of Industrial Microbiology and Biotechnology.
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Gosse, J.L., Chinn, M.S., Grunden, A.M. et al. A versatile method for preparation of hydrated microbial–latex biocatalytic coatings for gas absorption and gas evolution. J Ind Microbiol Biotechnol 39, 1269–1278 (2012). https://doi.org/10.1007/s10295-012-1135-8
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DOI: https://doi.org/10.1007/s10295-012-1135-8