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Shotgun proteomic monitoring of Clostridium acetobutylicum during stationary phase of butanol fermentation using xylose and comparison with the exponential phase

  • Short Communication
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Economically viable production of solvents through acetone–butanol–ethanol (ABE) fermentation requires a detailed understanding of Clostridium acetobutylicum. This study focuses on the proteomic profiling of C. acetobutylicum ATCC 824 from the stationary phase of ABE fermentation using xylose and compares with the exponential growth by shotgun proteomics approach. Comparative proteomic analysis revealed 22.9% of the C. acetobutylicum genome and 18.6% was found to be common in both exponential and stationary phases. The proteomic profile of C. acetobutylicum changed during the ABE fermentation such that 17 proteins were significantly differentially expressed between the two phases. Specifically, the expression of five proteins namely, CAC2873, CAP0164, CAP0165, CAC3298, and CAC1742 involved in the solvent production pathway were found to be significantly lower in the stationary phase compared to the exponential growth. Similarly, the expression of fucose isomerase (CAC2610), xylulose kinase (CAC2612), and a putative uncharacterized protein (CAC2611) involved in the xylose utilization pathway were also significantly lower in the stationary phase. These findings provide an insight into the metabolic behavior of C. acetobutylicum between different phases of ABE fermentation using xylose.

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Acknowledgments

The ORNL part of this research was sponsored in part by the U.S. Department of Energy under Contract DE-AC05-00OR22725 with Oak Ridge National Laboratory, managed and operated by UT-Battelle, LLC. We thank Dr. Tim Geary and Dr. Robert Kearney from McGill University for guiding with the proteomic data analysis and interpretation.

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Authors and Affiliations

  1. Department of Bioresource Engineering, Macdonald Campus, McGill University, Montreal, QC, Canada

    Kumaran Sivagnanam, Vijaya G. S. Raghavan & Mark G. Lefsrud

  2. Chemical and Life Sciences Divisions, Oak Ridge National Laboratory, Oak Ridge, TN, USA

    Manesh Shah, Robert L. Hettich & Nathan C. Verberkmoes

Authors
  1. Kumaran Sivagnanam
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  2. Vijaya G. S. Raghavan
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  3. Manesh Shah
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  4. Robert L. Hettich
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  5. Nathan C. Verberkmoes
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  6. Mark G. Lefsrud
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Corresponding author

Correspondence to Mark G. Lefsrud.

Electronic supplementary material

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10295_2012_1094_MOESM1_ESM.pdf

The proteins identified from C. acetobutylicum ATCC 824 and their functional classifications were shown in the supplementary tables. Supplementary material 1 (PDF 1,463 kb)

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Sivagnanam, K., Raghavan, V.G.S., Shah, M. et al. Shotgun proteomic monitoring of Clostridium acetobutylicum during stationary phase of butanol fermentation using xylose and comparison with the exponential phase. J Ind Microbiol Biotechnol 39, 949–955 (2012). https://doi.org/10.1007/s10295-012-1094-0

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  • DOI: https://doi.org/10.1007/s10295-012-1094-0

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