Abstract
The industrial Leuconostoc strain B/110-1-2 producing dextran and dextran derivatives was taxonomically identified by 16S rRNA as L. citreum. Its dextransucrase enzymes were characterized according to their cellular location and reaction specificity. In the presence of sucrose, the strain B/110-1-2 produced two cell-associated dextransucrases (31.54% of the total glucosyltransferase activity) with molecular weights of 160 and 240 kDa and a soluble dextransucrase (68.46%) at 160–180 kDa. Two open reading frames (ORF) coding for L. citreum strain B/110-1-2 dextransucrases were identified. One of them shared a 52% identity with the alternansucrase ASR of L. citreum NRRL B-1355 and with a putative annotated alternansucrase sequence found in the genome of L. citreum KM20. The structural analysis (HPAEC-PAD, HPSEC, and 13C-NMR) of the polymer and oligodextrans produced by the B/110-1-2 dextransucrases suggest this novel glucansucrase has specificity similar to a dextransucrase but not to an alternansucrase, producing a soluble linear dextran with glucose molecules linked mainly in α-1,6 and α-1,3 with α-1,4 branches. These results enhance the understanding of this industrially significant strain and will aid in distinguishing between physiologically similar Leuconostoc spp. strains.
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Acknowledgments
R. Fraga holds a contract as Research Associated from INRA, Toulouse. We express our gratitude to Elisse Champion and Richard Guyton by their technical support and advice for the HPAEC-PAD and HPSEC analysis, respectively. We also thank Dr. Joan Combie for the style correction of the manuscript.
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Fraga Vidal, R., Martínez, A., Moulis, C. et al. A novel dextransucrase is produced by Leuconostoc citreum strain B/110-1-2: an isolate used for the industrial production of dextran and dextran derivatives. J Ind Microbiol Biotechnol 38, 1499–1506 (2011). https://doi.org/10.1007/s10295-010-0936-x
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DOI: https://doi.org/10.1007/s10295-010-0936-x