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Post-translational processing of modular xylanases from Streptomyces is dependent on the carbohydrate-binding module

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Journal of Industrial Microbiology & Biotechnology

Abstract

Xylanases are very often modular enzymes composed of one or more catalytic domains and carbohydrate-binding modules (CBMs) connected by a flexible linker region. Usually, when these proteins are processed they lose their carbohydrate-binding capacity. Here, the role of the linker regions and cellulose- or xylan-binding domains in the processing of Xys1L from Streptomyces halstedii JM8 and Xyl30L from Streptomyces avermitilis UAH30 was studied. Xys1 variants with different linker lengths were tested, these being unable to avoid protein processing. Moreover, several fusion proteins between the Xys1 and Xyl30 domains were obtained and their proteolytic stability was studied. We demonstrate that CBM processing takes place even in the complete absence of the linker sequence. We also show that the specific carbohydrate module determines this cleavage in the proteins studied.

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Acknowledgments

This work was supported by grant CSI02A05 from the “Junta de Castilla y León” to R. Santamaría. Thanks are also due to MJ Jiménez Rufo for her excellent technical work, and to N. Skinner for supervising the English version of the manuscript.

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Authors and Affiliations

  1. Instituto de Microbiología Bioquímica/Departamento de Microbiología y Genética, CSIC/Universidad de Salamanca, Edificio Departamental, Campus Miguel de Unamuno, 37007, Salamanca, Spain

    Margarita Díaz, José M. Fernández-Ábalos & Ramón I. Santamaría

  2. Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Alcalá, Alcalá de Henares, Spain

    Juan Soliveri & José L. Copa-Patiño

Authors
  1. Margarita Díaz
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  2. José M. Fernández-Ábalos
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  3. Juan Soliveri
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  4. José L. Copa-Patiño
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  5. Ramón I. Santamaría
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Correspondence to Margarita Díaz.

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Díaz, M., Fernández-Ábalos, J.M., Soliveri, J. et al. Post-translational processing of modular xylanases from Streptomyces is dependent on the carbohydrate-binding module. J Ind Microbiol Biotechnol 38, 1419–1426 (2011). https://doi.org/10.1007/s10295-010-0927-y

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  • DOI: https://doi.org/10.1007/s10295-010-0927-y

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