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A novel methodology independent of fermentation rate for assessment of the fructophilic character of wine yeast strains

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

The yeast Saccharomyces cerevisiae has a fundamental role in fermenting grape juice to wine. During alcoholic fermentation its catabolic activity converts sugars (which in grape juice are a near equal ratio of glucose and fructose) and other grape compounds into ethanol, carbon dioxide and sensorily important metabolites. However, S. cerevisiae typically utilises glucose and fructose with different efficiency: glucose is preferred and is consumed at a higher rate than fructose. This results in an increasing difference between the concentrations of glucose and fructose during fermentation. In this study 20 commercially available strains were investigated to determine their relative abilities to utilise glucose and fructose. Parameters measured included fermentation duration and the kinetics of utilisation of fructose when supplied as sole carbon source or in an equimolar mix with glucose. The data were then analysed using mathematical calculations in an effort to identify fermentation attributes which were indicative of overall fructose utilisation and fermentation performance. Fermentation durations ranged from 74.6 to over 150 h, with clear differences in the degree to which glucose utilisation was preferential. Given this variability we sought to gain a more holistic indication of strain performance that was independent of fermentation rate and therefore utilized the area under the curve (AUC) of fermentation of individual or combined sugars. In this way it was possible to rank the 20 strains for their ability to consume fructose relative to glucose. Moreover, it was shown that fermentations performed in media containing fructose as sole carbon source did not predict the fructophilicity of strains in wine-like conditions (equimolar mixture of glucose and fructose). This work provides important information for programs which seek to generate strains that are faster or more reliable fermenters.

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Abbreviations

AUC:

Area under the curve

References

  1. Arroyo-Lopez FN, Querol A, Barrio E (2009) Application of a substrate inhibition model to estimate the effect of fructose concentration on the growth of diverse Saccharomyces cerevisiae strains. J Ind Microbiol Biotechnol 36:663–669

    Article  PubMed  CAS  Google Scholar 

  2. Berthels NJ, Cordero Otero RR, Bauer FF, Thevelein JM, Pretorius IS (2004) Discrepancy in glucose and fructose utilisation during fermentation by Saccharomyces cerevisiae wine yeast strains. FEMS Yeast Res 4:683–689

    Article  PubMed  CAS  Google Scholar 

  3. Berthels NJ, Cordero Otero RR, Bauer FF, Pretorius IS, Thevelein JM (2008) Correlation between glucose/fructose discrepancy and hexokinase kinetic properties in different Saccharomyces cerevisiae wine yeast strains. Appl Microbiol Biotechnol 77:1083–1091

    Article  PubMed  CAS  Google Scholar 

  4. Bisson LF, Block DE (2002) Ethanol tolerance in Saccharomyces. Biodivers Biotechnol Wine Yeast, 85–98

  5. Bisson LF, Fraenkel DG (1983) Involvement of kinases in glucose and fructose uptake by Saccharomyces cerevisiae. Proc Natl Acad Sci USA 80:1730–1734

    Article  PubMed  CAS  Google Scholar 

  6. Boehringe-Mannheim (1989) d-glucose/d-fructose. In: Methods of biochemical analysis and food analysis. Boehringer Mannheim, pp 50–55

  7. Cavazza A, Poznanski E, Trioli G (2004) Restart of fermentation of simulated stuck wines by direct inoculation of active dry yeasts. Am J Enol Vitic 55:160–167

    CAS  Google Scholar 

  8. Ciriacy M, Reifenberger E (1997) Hexose transport. In: Zimmermann FK, Entian KD (eds) Yeast sugar metabolism. Technimic, Lancaster, pp 45–65

    Google Scholar 

  9. Diderich JA, Schepper M, van Hoek P, Luttik MA, van Dijken JP, Pronk JT, Klaassen P, Boelens HF, de Mattos MJ, van Dam K, Kruckeberg AL (1999) Glucose uptake kinetics and transcription of HXT genes in chemostat cultures of Saccharomyces cerevisiae. J Biol Chem 274:15350–15359

    Article  PubMed  CAS  Google Scholar 

  10. Diderich JA, Schuurmans JM, Van Gaalen MC, Kruckeberg AL, Van Dam K (2001) Functional analysis of the hexose transporter homologue HXT5 in Saccharomyces cerevisiae. Yeast 18:1515–1524

    Article  PubMed  CAS  Google Scholar 

  11. Dumont A, Raynal C, Raginel F, Ortiz-Julien A (2009) The ability of wine yeast to consume fructose. Aust NZ Grapegrow Winemak 543:52–56

    Google Scholar 

  12. Elbing K, Larsson C, Bill RM, Albers E, Snoep JL, Boles E, Hohmann S, Gustafsson L (2004) Role of hexose transport in control of glycolytic flux in Saccharomyces cerevisiae. Appl Environ Microbiol 70:5323–5330

    Article  PubMed  CAS  Google Scholar 

  13. Gafner J, Schütz M (1996) Impact of glucose-fructose-ratio on stuck fermentations: practical experiences to restart stuck fermentations. Wein-Wissenschaft Vitic Enol Sci 51:214–218

    CAS  Google Scholar 

  14. Gonçalves P, Rodrigues de Sousa H, Spencer-Martins I (2000) FSY1, a novel gene encoding a specific fructose/H+ symporter in the type strain of Saccharomyces carlsbergensis. J Bacteriol 182:5628–5630

    Article  PubMed  Google Scholar 

  15. Guillaume C, Delobel P, Sablayrolles JM, Blondin B (2007) Molecular basis of fructose utilization by the wine yeast Saccharomyces cerevisiae: a mutated HXT3 allele enhances fructose fermentation. Appl Environ Microbiol 73:2432–2439

    Article  PubMed  CAS  Google Scholar 

  16. Henschke PA, Jiranek V (1993) Yeast—metabolism of nitrogen compound. In: Fleet GH (ed) Wine microbiology and biotechnology. Harwood Academic, Chur, pp 77–164

    Google Scholar 

  17. Jiranek V, Langridge P, Henschke PA (1995) Amino-acid and ammonium utilization by Saccharomyces cerevisiae wine yeasts from a chemically-defined medium. Am J Enol Vitic 46:75–83

    CAS  Google Scholar 

  18. Júnior MM, Batistote M, Ernandes JR (2008) Glucose and fructose fermentation by wine yeasts in media containing structurally complex nitrogen sources. J Inst Brew 114:199–204

    Google Scholar 

  19. Karpel JE, Place WR, Bisson LF (2008) Analysis of the major hexose transporter genes in wine strains of Saccharomyces cerevisiae. Am J Enol Vitic 59:265–275

    CAS  Google Scholar 

  20. Lee CK (1987) The chemistry and biochemistry of the sweetness of sugars. Adv Carbohydr Chem Biochem 45:199–351

    Article  PubMed  CAS  Google Scholar 

  21. Luyten K, Riou C, Blondin B (2002) The hexose transporters of Saccharomyces cerevisiae play different roles during enological fermentation. Yeast 19:713–726

    Article  PubMed  CAS  Google Scholar 

  22. McBryde C, Gardner JM, de Barros Lopes M, Jiranek V (2006) Generation of novel wine yeast strains by adaptive evolution. Am J Enol Vitic 57:423–430

    CAS  Google Scholar 

  23. Meneses FJ, Henschke PA, Jiranek V (2002) A survey of industrial strains of Saccharomyces cerevisiae reveals numerous altered patterns of maltose and sucrose utilisation. J Inst Brew 108:310–321

    CAS  Google Scholar 

  24. Meneses FJ, Jiranek V (2002) Expression patterns of genes and enzymes involved in sugar catabolism in industrial Saccharomyces cerevisiae strains displaying novel fermentation characteristics. J Inst Brew 108:322–335

    CAS  Google Scholar 

  25. Ozcan S, Johnston M (1995) Three different regulatory mechanisms enable yeast hexose transporter (HXT) genes to be induced by different levels of glucose. Mol Cell Biol 15:1564–1572

    PubMed  CAS  Google Scholar 

  26. Ozcan S, Johnston M (1999) Function and regulation of yeast hexose transporters. Microbiol Mol Biol Rev 63:554–569

    PubMed  CAS  Google Scholar 

  27. Perez M, Luyten K, Michel R, Riou C, Blondin B (2005) Analysis of Saccharomyces cerevisiae hexose carrier expression during wine fermentation: both low- and high-affinity Hxt transporters are expressed. FEMS Yeast Res 5:351–361

    Article  PubMed  CAS  Google Scholar 

  28. Ramos J, Szkutnicka K, Cirillo VP (1988) Relationship between low- and high-affinity glucose transport systems of Saccharomyces cerevisiae. J Bacteriol 170:5375–5377

    PubMed  CAS  Google Scholar 

  29. Reifenberger E, Freidel K, Ciriacy M (1995) Identification of novel HXT genes in Saccharomyces cerevisiae reveals the impact of individual hexose transporters on glycolytic flux. Mol Microbiol 16:157–167

    Article  PubMed  CAS  Google Scholar 

  30. Rolland F, Wanke V, Cauwenberg L, Ma PS, Boles E, Vanoni M, de Winde JH, Thevelein JM, Winderickx J (2001) The role of hexose transport and phosphorylation in cAMP signalling in the yeast Saccharomyces cerevisiae. FEMS Yeast Res 1:33–45

    PubMed  CAS  Google Scholar 

  31. Rolland F, Winderickx J, Thevelein JM (2001) Glucose-sensing mechanisms in eukaryotic cells. Trends Biochem Sci 26:310–317

    Article  PubMed  CAS  Google Scholar 

  32. Salmon J-M (1989) Effects of sugar transport inactivation in Saccharomyces cerevisiae on sluggish and stuck enological fermentations. Appl Environ Microbiol 55:953–958

    PubMed  CAS  Google Scholar 

  33. Salmon JM, Vincent O, Mauricio JC, Bely M, Barre P (1993) Sugar-transport inhibition and apparent loss of activity in Saccharomyces cerevisiae as a major limiting factor of enological fermentations. Am J Enol Vitic 44:56–64

    CAS  Google Scholar 

  34. Serrano R, Delafuente G (1974) Regulatory properties of the constitutive hexose transport in Saccharomyces cerevisiae. Mol Cell Biochem 5:161–171

    Article  PubMed  CAS  Google Scholar 

  35. Stanley D, Bandara A, Fraser S, Chambers PJ, Stanley GA (2010) The ethanol stress response and ethanol tolerance of Saccharomyces cerevisiae. J Appl Microbiol. (doi: 10.1111/j.1365-2672.2009.04657.x)

  36. Tronchoni J, Gamero A, Arroyo-Lopez FN, Barrio E, Querol A (2009) Differences in the glucose and fructose consumption profiles in diverse Saccharomyces wine species and their hybrids during grape juice fermentation. Int J Food Microbiol 134:237–243

    Article  PubMed  CAS  Google Scholar 

  37. Varela C, Cardenas J, Melo F, Agosin E (2005) Quantitative analysis of wine yeast gene expression profiles under winemaking conditions. Yeast 22:369–383

    Article  PubMed  CAS  Google Scholar 

  38. Verwaal R, Paalman JW, Hogenkamp A, Verkleij AJ, Verrips CT, Boonstra J (2002) HXT5 expression is determined by growth rates in Saccharomyces cerevisiae. Yeast 19:1029–1038

    Article  PubMed  CAS  Google Scholar 

  39. Wang D, Xu Y, Hu J, Zhao G (2004) Fermentation kinetics of different sugars by apple wine yeast Saccharomyces cerevisiae. J Inst Brew Distill 110:340–346

    CAS  Google Scholar 

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Authors and Affiliations

  1. School of Agriculture, Food and Wine, The University of Adelaide, PMB 1, Glen Osmond, SA, 5064, Australia

    T. Liccioli & V. Jiranek

  2. Wine Innovation Cluster, Adelaide, SA, Australia

    T. Liccioli, P. J. Chambers & V. Jiranek

  3. The Australian Wine Research Institute, PO Box 197, Glen Osmond, SA, 5064, Australia

    P. J. Chambers

Authors
  1. T. Liccioli
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  2. P. J. Chambers
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  3. V. Jiranek
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Correspondence to V. Jiranek.

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Liccioli, T., Chambers, P.J. & Jiranek, V. A novel methodology independent of fermentation rate for assessment of the fructophilic character of wine yeast strains. J Ind Microbiol Biotechnol 38, 833–843 (2011). https://doi.org/10.1007/s10295-010-0854-y

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  • DOI: https://doi.org/10.1007/s10295-010-0854-y

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