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Enzyme replacement therapy for Morquio A: an active recombinant N-acetylgalactosamine-6-sulfate sulfatase produced in Escherichia coli BL21

  • Original Paper
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Journal of Industrial Microbiology & Biotechnology

Abstract

Mucopolysaccharidosis IVA (MPS IVA) is an autosomal recessive disorder caused by N-acetylgalactosamine-6-sulfate sulfatase (GALNS) deficiency. Currently no effective therapies exist for MPS IVA. In this work, production of a recombinant GALNS enzyme (rGALNS) in Escherichia coli BL21 strain was studied. At shake scale, the effect of glucose concentration on microorganism growth, and microorganism culture and induction times on rGALNS production were evaluated. At bench scale, the effect of aeration and agitation on microorganism growth, and culture and induction times were evaluated. The highest enzyme activity levels at shake scale were observed in 12 h culture after 2–4 h induction. At bench scale the highest enzyme activity levels were observed after 2 h induction. rGALNS amounts in inclusion bodies fraction were up to 17-fold higher than those observed in the soluble fraction. However, the highest levels of active enzyme were found in the soluble fraction. Western blot analysis showed the presence of a 50-kDa band, in both soluble and inclusion bodies fractions. These results show for the first time the feasibility and potential of production of active rGALNS in a prokaryotic system for development of enzyme replacement therapy for MPS IVA disease.

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Acknowledgments

We thank Dr. Shunji Tomatsu and Dr. Adriana Montaño (Saint Louis University) for pCXN-GALNS plasmid, recombinant human GALNS enzyme, and anti-GALNS monoclonal antibody. We also thank Dr. Patricia Landazuri (Universidad del Quindio) for pGEX-3X expression plasmid, Dr. Raul Poutou (Pontificia Universidad Javeriana) for critical revision of the manuscript, and Carlos Soto for his assistance.

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Authors and Affiliations

  1. Institute for the Study of Inborn Errors of Metabolism, School of Sciences, Pontificia Universidad Javeriana, Kra 7 No. 43-82 Building 53, Room 305A, Bogotá, Colombia

    Alexander Rodríguez, Ángela J. Espejo, Lina M. Lizaraso, Henry A. Cordoba, Carlos J. Alméciga-Díaz & Luis A. Barrera

  2. Department of Chemical Engineering, Group of Process and Products Design, Universidad de Los Andes, Kra 1 E No. 19 A-40, Bogotá, Colombia

    Alejandra Hernández, Olga L. Velásquez & Oscar F. Sánchez

  3. Chemical Department, School of Sciences, Pontificia Universidad Javeriana, Bogotá, Colombia

    Henry A. Cordoba

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  1. Alexander Rodríguez
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  2. Ángela J. Espejo
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  3. Alejandra Hernández
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  4. Olga L. Velásquez
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  5. Lina M. Lizaraso
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  7. Oscar F. Sánchez
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  9. Luis A. Barrera
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Correspondence to Oscar F. Sánchez or Carlos J. Alméciga-Díaz.

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Rodríguez, A., Espejo, Á.J., Hernández, A. et al. Enzyme replacement therapy for Morquio A: an active recombinant N-acetylgalactosamine-6-sulfate sulfatase produced in Escherichia coli BL21. J Ind Microbiol Biotechnol 37, 1193–1201 (2010). https://doi.org/10.1007/s10295-010-0766-x

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