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Enhanced d-ribose biosynthesis in batch culture of a transketolase-deficient Bacillus subtilis strain by citrate

  • Original Paper
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Journal of Industrial Microbiology & Biotechnology

Abstract

In this study, the effects of citrate addition on d-ribose production were investigated in batch culture of a transketolase-deficient strain, Bacillus subtilis EC2, in shake flasks and bioreactors. Batch cultures in shake flasks and a 5-l reactor indicated that supplementation with 0.2–0.5 g l−1 of citrate enhanced d-ribose production. When B. subtilis EC2 was cultivated in a 15-l reactor in a complex medium, the d-ribose concentration was 70.9 g l−1 with a ribose yield of 0.497 mol mol−1. When this strain was grown in the same medium supplemented with 0.3 g l−1 of citrate, 83.4 g l−1 of d-ribose were obtained, and the ribose yield was increased to 0.587 mol mol−1. Addition of citrate reduced the activities of pyruvate kinase and phosphofructokinase, while it increased those of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase. Metabolic flux distribution in the stationary phase indicated that citrate addition resulted in increased fluxes in the pentose phosphate pathway and TCA cycle, and decreased fluxes in the glycolysis and acetate pathways.

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Acknowledgment

This work was partly supported by the Project of Shanghai Leading Academic Disciplines, no. B505.

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  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, China

    Lin Wu, Zhimin Li & Qin Ye

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  1. Lin Wu
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  2. Zhimin Li
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Correspondence to Qin Ye.

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Wu, L., Li, Z. & Ye, Q. Enhanced d-ribose biosynthesis in batch culture of a transketolase-deficient Bacillus subtilis strain by citrate. J Ind Microbiol Biotechnol 36, 1289–1296 (2009). https://doi.org/10.1007/s10295-009-0612-1

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  • DOI: https://doi.org/10.1007/s10295-009-0612-1

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