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Studies on the production of nigerloxin using agro-industrial residues by solid-state fermentation

Journal of Industrial Microbiology & Biotechnology volume 36, pages 1179–1187 (2009)Cite this article

Abstract

Nigerloxin, a new and potent lipoxygenase inhibitor, was discovered in our laboratory through solid-state fermentation of wheat bran by Aspergillus niger V. Teigh (MTCC-5166). The aim of this study is to investigate the possibility of using different agro-industrial residues as nutritional supplements along with wheat bran to enhance the production of nigerloxin. Nigerloxin produced by SSF was quantified spectrophotometrically at 292 nm. The results indicate that the inhibitor production was influenced by the type of solid substrate supplemented, moisture content, pH and size of the inoculum. Individually optimized supplements were tested in different combinations to determine their effects on nigerloxin production. A twofold increase in the production of nigerloxin (4.9 ± 0.3 mg gds−1) was achieved by supplementing wheat bran with 10% w/w sweet lemon peel and 5% v/w methanol at optimized process parameters, that is, an initial moisture content of 65% v/w and incubation period of 6 days with an initial inoculum size of 2 ml (8 × 105 spores gds−1). Nigerloxin production was stable between pH of 4 and 5.

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Acknowledgments

The authors thank the Department of Biotechnology, New Delhi, India, for financial support, and the Director, CFTRI, for facilities. DC thanks the Council of Scientific and Industrial Research, New Delhi, India, for the Internship Fellowship.

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  1. Fermentation Technology and Bio Engineering Department, Central Food Technological Research Institute, Mysore, 570020, India

    Dasagrandhi Chakradhar, Saleem Javeed & Avinash P. Sattur

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  1. Dasagrandhi Chakradhar
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  2. Saleem Javeed
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  3. Avinash P. Sattur
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Correspondence to Avinash P. Sattur.

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Chakradhar, D., Javeed, S. & Sattur, A.P. Studies on the production of nigerloxin using agro-industrial residues by solid-state fermentation. J Ind Microbiol Biotechnol 36, 1179–1187 (2009). https://doi.org/10.1007/s10295-009-0599-7

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  • DOI: https://doi.org/10.1007/s10295-009-0599-7

Keywords

  • Agro-industrial residues
  • Solid-state fermentation
  • Aspergillus niger V. Teigh (MTCC-5166)
  • Nigerloxin
  • Lipoxygenase inhibitor