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Optimized lipase-catalyzed synthesis of adipate ester in a solvent-free system

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Journal of Industrial Microbiology & Biotechnology


Immobilized Candida antarctica lipase-catalyzed esterification of adipic acid and oleyl alcohol was investigated in a solvent-free system (SFS). Optimum conditions for adipate ester synthesis in a stirred-tank reactor were determined by the response surface methodology (RSM) approach with respect to important reaction parameters including time, temperature, agitation speed, and amount of enzyme. A high conversion yield was achieved using low enzyme amounts of 2.5% w/w at 60°C, reaction time of 438 min, and agitation speed of 500 rpm. The good correlation between predicted value (96.0%) and actual value (95.5%) implies that the model derived from RSM allows better understanding of the effect of important reaction parameters on the lipase-catalyzed synthesis of adipate ester in an organic solvent-free system. Higher volumetric productivity compared to a solvent-based system was also offered by SFS. The results demonstrate that the solvent-free system is efficient for enzymatic synthesis of adipate ester.

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Correspondence to Mohd Basyaruddin Abdul Rahman.

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Chaibakhsh, N., Abdul Rahman, M.B., Abd-Aziz, S. et al. Optimized lipase-catalyzed synthesis of adipate ester in a solvent-free system. J Ind Microbiol Biotechnol 36, 1149–1155 (2009).

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