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Production of fructosyltransferase by Aureobasidium sp. ATCC 20524 in batch and two-step batch cultures

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Journal of Industrial Microbiology & Biotechnology

Abstract

A comparison of fructosyltransferase (EC 2.4.1.9) production by Aureobasidium sp. ATCC 20524 in batch and two step batch cultures was investigated in a 1-l stirred tank reactor using a sucrose supply of 200 g/l. Results showed that the innovative cultivation in two step of Aureobasidium sp. produced more fructosyltransferase (FFase) than the single batch culture at the same sucrose concentration with a maximal enzyme production of 523 U/ml, which was 80.5% higher than the one obtained in the batch culture. The production of fructooligosaccharides (FOSs) was also analyzed; their concentration reached a maximum value of 160 g/l the first day in the two-step culture and 127 g/l in the single-batch mode. The use of the two-step batch culture with Aureobasidium sp. ATCC 20524 in allowing the microorganism to grow up prior to the induction of sucrose (second step), proved to be a powerful method for producing fructosyltransferase and FOSs.

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Acknowledgments

This work was supported by differents grants from ANPCyT (Agencia Nacional de Promoción Científica y Tecnológica), CIUNT (Consejo de Investigaciones de la Universidad Nacional de Tucumán) and CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas). The authors thank Dr Ricardo Fitzsimons for his help with HPLC assays and Dr Alejanda Martinez for helpful discussion.

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Authors and Affiliations

  1. PROIMI-CONICET (Planta Piloto de Procesos Industriales Microbiológicos, Consejo Nacional de Investigaciones Científicas y Tecnológicas), Av. Belgrano y Pje. Caseros, San Miguel de Tucumán, CP, 4000, Argentina

    Martín A. Salinas & Nora I. Perotti

  2. Cátedra de Microbiología General e Industrial, Facultad de Ciencias Exactas y Tecnología, Universidad Nacional de Tucumán, Tucumán, Argentina

    Martín A. Salinas & Nora I. Perotti

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  1. Martín A. Salinas
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  2. Nora I. Perotti
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Correspondence to Martín A. Salinas.

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Salinas, M.A., Perotti, N.I. Production of fructosyltransferase by Aureobasidium sp. ATCC 20524 in batch and two-step batch cultures. J Ind Microbiol Biotechnol 36, 39–43 (2009). https://doi.org/10.1007/s10295-008-0470-2

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  • DOI: https://doi.org/10.1007/s10295-008-0470-2

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