Abstract
The treatment of chickpea milk was carried out in batch, repeated batch and continuous reaction by soluble and polyvinyl alcohol (PVA) immobilized Aspergillus oryzae α-galactosidase for the removal of raffinose family oligosaccharides (RFOs). In the batch mode of treatment 96 and 92% of RFOs hydrolysis was observed by soluble and immobilized enzyme, respectively. In repeated batch experiments, immobilized enzyme showed 70% RFOs hydrolysis up to sixth cycle. Polyvinyl alcohol immobilized α-galactosidase in fluidized bed reactor showed highest reduction of 94% at a flow rate of 30 ml/h. The results obtained from the present study are very interesting for industrial use of PVA-immobilized enzyme.
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Mr. Aravind Goud Patil thanks Gulbarga University, Gulbarga, India for awarding meritorious PhD Junior Research Fellowship.
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Patil, A.G.G., Kote, N.V. & Mulimani, V. Enzymatic removal of flatulence-inducing sugars in chickpea milk using free and polyvinyl alcohol immobilized α-galactosidase from Aspergillus oryzae . J Ind Microbiol Biotechnol 36, 29–33 (2009). https://doi.org/10.1007/s10295-008-0467-x
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DOI: https://doi.org/10.1007/s10295-008-0467-x