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Production of laccases in submerged process by Pleurotus sajor-caju PS-2001 in relation to carbon and organic nitrogen sources, antifoams and Tween 80

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Journal of Industrial Microbiology & Biotechnology

Abstract

Some conditions in media composition for laccases production, such as different sources of carbon and organic nitrogen, antifoams and a surfactant, were studied in liquid cultures of Pleurotus sajor-caju strain PS-2001. Cultivation with fructose or glucose as carbon sources produced maximum enzyme activities of 37 and 36 U mL−1, respectively. When sucrose was present in the medium, the best results were obtained using 5 g L−1 of this carbohydrate, on the 11th day of the process, attaining laccase titres of 13 U mL−1. In a medium without casein, practically no enzyme was produced during the experiments; among the sources of nitrogen studied, pure casein led to the highest titres of laccase activity. Different concentrations of pure casein and sucrose were also tested. As to the different concentrations of casein, the addition of 1.5 g L−1 resulted in the highest titres of laccase activity. Negligible levels of manganese peroxidase activity were also detected in the culture medium. In low concentrations, polypropylene glycol or silicon-based antifoams and the surfactant Tween 80 have no significant influence on the formation of laccases by P. sajor-caju. However, enhanced concentration of polypropylene glycol negatively affected the production of laccases but favored the titres in total peroxidases, lignin peroxidase and veratryl alcohol oxidase.

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Acknowledgments

This study was supported by Fundação de Amparo à Pesquisa do Rio Grande do Sul (FAPERGS) and University of Caxias do Sul (UCS). F. Bettin receives a scholarship from the Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil).

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  1. Institute of Biotechnology, University of Caxias do Sul, Rua Francisco Getulio Vargas 1130, Caxias do Sul, RS, 95070-560, Brazil

    Fernanda Bettin, Queli Montanari, Raquel Calloni, Tamara A. Gaio, Mauricio M. Silveira & Aldo J. P. Dillon

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  1. Fernanda Bettin
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Bettin, F., Montanari, Q., Calloni, R. et al. Production of laccases in submerged process by Pleurotus sajor-caju PS-2001 in relation to carbon and organic nitrogen sources, antifoams and Tween 80. J Ind Microbiol Biotechnol 36, 1–9 (2009). https://doi.org/10.1007/s10295-008-0463-1

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