Abstract
Efficacy of culture filtrates of five strains of Metarhizium anisopliae isolated from insects were evaluated against Anopheles stephensi and Culex quinquefasciatus. The culture filtrates released from the strains of M. anisopliae in the YpSs and chitin broths were filtered and used for the bioassays after a growth of 7 days. Among the culture filtrates of five strains, M. anisopliae 892 was found to be more effective against both the mosquitoes. The LC50 values of culture filtrates of M. anisopliae 892 in chitin broth was lower than the LC50 of culture filtrates in YpSs broth against first and fourth instars of both the mosquitoes. The LC50 values of culture filtrates were significantly different between first and fourth instars of A. stephensi (t test; P = 0.0001) and C. quinquefasciatus (t test; P = 0.02). The larvae of A. stephensi were more susceptible than C. quinquefasciatus except in two cases. This is the first report of efficacy of culture filtrates produced by M. anisopliae in chitin broth against mosquitoes and have potential as a biological control agent of mosquitoes.
This is a preview of subscription content, log in via an institution to check access.
References
World Health Organization (1993) Implementation of the global malaria control strategy, WHO Tech Rep Ser 839
Das PK, Pani SP (2002) Filariasis in India; epidemiology and control. In: Sood ML (ed) Helminthology in India. International Book Distributors, Dhera Dun, pp 425–429
Sharma VP (1998) Fighting malaria in India. Curr Sci 75:1127–1140
Quesada-Moraga E, Carrasco-Dıaz JA, Santiago-Alvarez C (2006) Insecticidal and antifeedant activities of proteins secreted by entomopathogenic fungi against Spodoptera littoralis (Lep., Noctuidae). J Appl Entomol 130:442–452. doi:10.1111/j.1439-0418.2006.01079.x
Roberts DW (1981) Toxins of entomopathogenic fungi. In: Burges HD (ed) Microbial control of pests and plant disease. Academic Press, New York, pp 441–464
Vey A, Hoagland R, Butt TM (2001) Toxic metabolites of fungal biocontrol agents. In: Butt TM, Jackson CW, Magan N (eds) Fungi as biocontrol agents progress: problems and potential. CABI Publishing, Wallingford, pp 311–346
Quesada-Moraga E, Vey A (2003) Intra-specific variation in virulence and in vitro production of macromolecular toxins active against locust among Beauveria bassiana strains, and effects of in vivo and in vitro passages on these factors. Biocontrol Sci Technol 13:323–340. doi:10.1080/0958315031000110346
Shimahara K, Takiguchi Y (1988) Preparation of crustacean chitin. Methods Enzymol 161(B):417
Abbott WS (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol 18:265–267
St Leger RS, Screen S (2001) Prospects for strain improvement of fungal pathogens of insects and weeds. In: Butt TM, Jackson CW, Magan N (eds) Fungi as biocontrol agents progress: problems and potential. CABI Publishing, Wallingford, pp 219–238
Roberts DW (1966) Toxins from the entomogenous fungus Metarhizium anisopliae. 1. Production in submerged and surface cultures, and in inorganic and organic nitrogen media. J Invertebr Pathol 8:212–221. doi:10.1016/0022-2011(66)90131-5
Vijayan V, Balaraman K (1991) Metabolites of fungi and actinomycetes active against mosquito larvae. Indian J Med Res 93:115–117
Mohanty SS, Prakash S (2004) Extracellular metabolites of Trichophyton ajelloi against Anopheles stephensi and Culex quinquefasciatus larvae. Curr Sci 86:323–325
Vyas N, Dua K, Prakash S (2007) Efficacy of Lagenidium giganteum metabolites on mosquito larvae with reference to nontarget organisms. Parasitol Res 101:385–390. doi:10.1007/s00436-007-0496-9
Acknowledgments
The first author acknowledges the Council of Scientific and Industrial Research, New Delhi, for financial support in the form of Research Associateship.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
10295_2008_434_MOESM1_ESM.doc
Supplementary Table. pH of the broths, culture filtrates of M. anisopliae 892 after growth of 7 days and final test medium at a concentration of 100 µL/mL (DOC 25 kb)
Rights and permissions
About this article
Cite this article
Mohanty, S.S., Raghavendra, K., Mittal, P.K. et al. Efficacy of culture filtrates of Metarhizium anisopliae against larvae of Anopheles stephensi and Culex quinquefasciatus . J Ind Microbiol Biotechnol 35, 1199–1202 (2008). https://doi.org/10.1007/s10295-008-0434-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10295-008-0434-6