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Effect of different cultural conditions for phytase production by Aspergillus niger CFR 335 in submerged and solid-state fermentations

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

The present article deals with the studies on the effect of media ingredients, such as carbon, nitrogen, inorganic phosphates, surfactants, and metal salts, on phytase enzyme production by Aspergillus niger CFR 335 in submerged (SmF) and solid-state fermentations (SSF). The results obtained showed a 1.5-fold higher enzyme yield in the presence of sucrose in both SmF and SSF, while peptone was found to be a favorable nitrogen source for SmF. Sodium dihydrogen phosphate (NaH2PO4) favored 34% higher enzyme yield than the control, which was followed by 19% higher activity in potassium dihydrogen phosphate (KH2PO4) in SSF at 0.015% w/v. The addition of Tween-20 in SmF showed a maximum yield of 12.6 U/mL while, SDS suppressed the growth of the fungus. None of the surfactants favored the enzyme yield in SSF. Calcium chloride (CaCl2) was extensively efficient in stimulating more than 55% higher phytase production in SmF at 0.01% v/v. In SSF, none of the metal salts stimulated phytase production.

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Acknowledgments

Authors are thankful to the Director, Central Food Technological Research Institute, Head, Food Microbiology Department, for his constant support and encouragement to carry out this research and University of Mysore for the financial aid.

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  1. Food Microbiology Department, Central Food Technological Research Institute, Mysore, 570 020, India

    B. S. Gunashree & G. Venkateswaran

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Correspondence to G. Venkateswaran.

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Gunashree, B.S., Venkateswaran, G. Effect of different cultural conditions for phytase production by Aspergillus niger CFR 335 in submerged and solid-state fermentations. J Ind Microbiol Biotechnol 35, 1587–1596 (2008). https://doi.org/10.1007/s10295-008-0402-1

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