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Effects of lipidic carbon sources on the extracellular lipolytic activity of a newly isolated strain of Bacillus subtilis

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Journal of Industrial Microbiology & Biotechnology

Abstract

An isolate exhibiting high extracellular lipolytic activity was identified as Bacillus subtilis by 16S rRNA gene sequence analysis. The enzyme activity of the isolate was improved by using different concentrations of lipidic carbon sources such as vegetable oils, fatty acids and triglycerides. Lipolytic activity was assayed spectrophotometrically using p-nitrophenyl palmitate. One percent (v/v) of sesame oil provided the highest activity with 80 and 98% enhancements with respect to 1% (v/v) concentrations of linoleic acid and triolein as the favored fatty acid and triglyceride, respectively. Glucose presented a repressive effect on lipase production. Lipase secreted by B. subtilis was partially purified by ultrafiltration and anion exchange chromatography; and the purified enzyme was tested for its residual activity in the presence of EDTA, SDS, Triton X-100, Tween 20, Tween 80 and protease. The present work reports, for the first time, that the lipolytic activity of a B. subtilis strain can be improved by using inexpensive vegetable oils; and also that B. subtilis lipase is suitable for use in detergents.

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Acknowledgments

The authors gratefully acknowledge the financial supports provided by Ankara University Biotechnology Institute (Project No: 2005-164 of the SPO Project No: 2001K120240) and by Ankara University Scientific Research Projects Unit (Project No: 2007-0745004-HPD). The suggestions and experimental support from Prof. Dr. G. Dönmez and Sevgi Ertuğrul (Ankara University Department of Biology) on the isolation of microorganism are gratefully recognized. Sincere thanks are extended to Assoc. Prof. Dr. H. Özdağ and G. Özsöz (Ankara University Biotechnology Institute) for their technical and experimental assistances on 16S rRNA gene sequencing, respectively. Analyses for fatty acid compositions of vegetable oils were kindly performed by Prof. Dr. A. Tekin (Ankara University, Department of Food Engineering).

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Authors and Affiliations

  1. Department of Chemical Engineering, Faculty of Engineering, Ankara University, 06100, Tandogan, Ankara, Turkey

    Serpil Takaç

  2. Institute of Biotechnology, Ankara University, 06100, Tandogan, Ankara, Turkey

    Serpil Takaç & Başak Marul

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  1. Serpil Takaç
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  2. Başak Marul
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Correspondence to Serpil Takaç.

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Takaç, S., Marul, B. Effects of lipidic carbon sources on the extracellular lipolytic activity of a newly isolated strain of Bacillus subtilis . J Ind Microbiol Biotechnol 35, 1019–1025 (2008). https://doi.org/10.1007/s10295-008-0377-y

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  • DOI: https://doi.org/10.1007/s10295-008-0377-y

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