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High cell density cultivation of Escherichia coli with surface anchored transglucosidase for use as whole-cell biocatalyst for α-arbutin synthesis

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Journal of Industrial Microbiology & Biotechnology


A fed-batch culture strategy for the production of recombinant Escherichia coli cells anchoring surface-displayed transglucosidase for use as a whole-cell biocatalyst for α-arbutin synthesis was developed. Lactose was used as an inducer of the recombinant protein. In fed-batch cultures, dissolved oxygen was used as the feed indicator for glucose, thus accumulation of glucose and acetate that affected the cell growth and recombinant protein production was avoided. Fed-batch fermentation with lactose induction yielded a biomass of 18 g/L, and the cells possessed very high transglucosylation activity. In the synthesis of α-arbutin by hydroquinone glucosylation, the whole-cell biocatalysts showed a specific activity of 501 nkat/g cell and produced 21 g/L of arbutin, which corresponded to 76% molar conversion. A sixfold increased productivity of whole cell biocatalysts was obtained in the fed-batch culture with lactose induction, as compared to batch culture induced by IPTG.

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This research was supported by grants (NSC 94-2214-E-0016-016) and (NSC 95-2221-E-006-304) from National Science Council of Taiwan.

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Correspondence to Wen-Teng Wu.

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Wu, PH., Nair, G.R., Chu, IM. et al. High cell density cultivation of Escherichia coli with surface anchored transglucosidase for use as whole-cell biocatalyst for α-arbutin synthesis. J Ind Microbiol Biotechnol 35, 95–101 (2008).

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