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Antifouling activity of sessile bacilli derived from marine surfaces

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Marine biofilms are a virtually untapped source of bioactive molecules that may find application as novel antifoulants in the marine paint industry. This study aimed at determining the potential of marine biofilm bacteria to produce novel biomolecules with potential application as natural antifoulants. Nine representative strains were isolated from a range of surfaces and were grown in YEB medium and harvested during the late exponential growth phase. Bacterial biomass and spent culture medium were extracted with ethanol and ethyl acetate, respectively. Extracts were assayed for their antifouling activity using two tests: (1) antimicrobial well diffusion test against a common fouling bacterium, Halomonas marina, and (2) anti-crustacean activity test using Artemia salina. Our results showed that none of the ethanolic extracts (bacterial biomass) were active in either test. In contrast, most of the organic extracts had antimicrobial activity (88%) and were toxic towards A. salina (67%). Sequencing of full 16 S ribosomal DNA analysis showed that the isolates were related to Bacillus mojavensis and Bacillus firmus. Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF-MS) profiling of ethyl acetate extracts of culture supernatants showed that these species produce the bioactive lipopeptides surfactin A, mycosubtilin and bacillomycin D.

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Acknowledgements

This research was supported by the international Foundation for Science, Stockholm, Sweden, and the Organisation for the Prohibition of Chemical Weapons, The Hague, The Netherlands, through a grant F/3617-1 to B.O.O.M. T.S is grateful for unrestricted access to MALDI-TOF MS equipement at the Institut für Molekulare Biowissenschaften, Universität Frankfurt am Main, Germany.

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Authors and Affiliations

  1. Departamento de Recursos del Mar, CINVESTAV Unidad Mérida, carretera antigua a Progreso Km. 6, Cordemex C.P. 97310, Mérida, Yucatán, Mexico

    Benjamín Otto Ortega-Morales

  2. Departamento de Microbiología Ambiental y Biotecnología, Universidad Autónoma de Campeche, Av. Agustín Melgar s/n, Col. Buenavista, C.P. 24030, Campeche, Mexico

    Benjamín Otto Ortega-Morales & Manuel Jesús Chan-Bacab

  3. Departamento de Biotecnología Ambiental, Ecología Microbiana Aplicada y Contaminación, El Colegio de la Frontera Sur, Unidad Chetumal, Av. del Centenario km 5.5, Col. Calderitas, C.P. 77900, Chetumal, Quintana Roo, Mexico

    Elizabeth Miranda-Tello

  4. Institute de Recherche pour le Developpement, UR-101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL case 925, 163 avenue de Luminy, 13288, Marseille cedex 09, France

    Marie-Laure Fardeau

  5. Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico DF, Mexico

    Julio César Carrero

  6. Institut für Molekulare Biowissenschaften, JW Goethe, Universität Frankfurt am Main, Max-von-Laue-Str, 960438, Frankfurt, Germany

    Torsten Stein

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  1. Benjamín Otto Ortega-Morales
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  2. Manuel Jesús Chan-Bacab
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  3. Elizabeth Miranda-Tello
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  4. Marie-Laure Fardeau
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  5. Julio César Carrero
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  6. Torsten Stein
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Correspondence to Benjamín Otto Ortega-Morales.

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Ortega-Morales, B.O., Chan-Bacab, M.J., Miranda-Tello, E. et al. Antifouling activity of sessile bacilli derived from marine surfaces. J Ind Microbiol Biotechnol 35, 9–15 (2008). https://doi.org/10.1007/s10295-007-0260-2

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  • DOI: https://doi.org/10.1007/s10295-007-0260-2

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