Production of laccase by a newly isolated deuteromycete fungus Pestalotiopsis sp. and its decolorization of azo dye

  • Jiejie Hao
  • Fuqiang Song
  • Feng Huang
  • Changlin Yang
  • Zhijun Zhang
  • Yi Zheng
  • Xingjun TianEmail author
Original Paper


The effect of various carbon and nitrogen sources on the production of laccase by newly isolated deuteromycete Pestalotiopsis sp. was tested under liquid-state fermentation. Twenty grams per liter of glucose and 10 g l−1 ammonium tartrate were found to be the optimized concentrations of carbon and nitrogen sources, respectively. The influence of different inducers and inhibitors on the laccase production was also examined. Adding the Cu up to optimum concentration of 2.0 mM in medium (include 20 g l−1 glucose and 10 g l−1 ammonium tartrate), the highest laccase activity of 32.7 ± 1.7 U ml−l was achieved. Cu had to be supplemented after 2 days of growth for its maximal effect, an addition after 6 days of growth, during which laccase activity was dominantly formed, resulted in distinctly reduced laccase activity. In addition, Direct Fast Blue B2RL can be effectively decolorized by crude laccase, the decolorization percentage of which was 88.0 ± 3.2% at pH 4.0 within 12 h. The results suggest that Pestalotiopsis sp. is a high potential producer of the industrially important enzyme laccase.


Deuteromycete Laccase Inducers Decolorization Azo dye 



The experiment was supported by National Natural Science Foundation of China (30470299), the Open Research Foundation of Key Laboratory for Modern Sylvicultural Technology of Zhejiang Province, Zhejiang Forestry College, China, and Key Project of National Science Foundation of China (30430570).


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Copyright information

© Society for Industrial Microbiology 2006

Authors and Affiliations

  • Jiejie Hao
    • 1
  • Fuqiang Song
    • 1
    • 2
  • Feng Huang
    • 1
  • Changlin Yang
    • 1
  • Zhijun Zhang
    • 1
    • 2
  • Yi Zheng
    • 1
  • Xingjun Tian
    • 1
    Email author
  1. 1.School of Life ScienceNanjing UniversityNanjingChina
  2. 2.Key Laboratory for Modern Sylvicultural Technology of Zhejiang ProvinceZhejiang Forestry CollegeZhejiangChina

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