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Production of laccase by a newly isolated deuteromycete fungus Pestalotiopsis sp. and its decolorization of azo dye

  • Jiejie Hao
  • Fuqiang Song
  • Feng Huang
  • Changlin Yang
  • Zhijun Zhang
  • Yi Zheng
  • Xingjun TianEmail author
Original Paper

Abstract

The effect of various carbon and nitrogen sources on the production of laccase by newly isolated deuteromycete Pestalotiopsis sp. was tested under liquid-state fermentation. Twenty grams per liter of glucose and 10 g l−1 ammonium tartrate were found to be the optimized concentrations of carbon and nitrogen sources, respectively. The influence of different inducers and inhibitors on the laccase production was also examined. Adding the Cu up to optimum concentration of 2.0 mM in medium (include 20 g l−1 glucose and 10 g l−1 ammonium tartrate), the highest laccase activity of 32.7 ± 1.7 U ml−l was achieved. Cu had to be supplemented after 2 days of growth for its maximal effect, an addition after 6 days of growth, during which laccase activity was dominantly formed, resulted in distinctly reduced laccase activity. In addition, Direct Fast Blue B2RL can be effectively decolorized by crude laccase, the decolorization percentage of which was 88.0 ± 3.2% at pH 4.0 within 12 h. The results suggest that Pestalotiopsis sp. is a high potential producer of the industrially important enzyme laccase.

Keywords

Deuteromycete Laccase Inducers Decolorization Azo dye 

Notes

Acknowledgments

The experiment was supported by National Natural Science Foundation of China (30470299), the Open Research Foundation of Key Laboratory for Modern Sylvicultural Technology of Zhejiang Province, Zhejiang Forestry College, China, and Key Project of National Science Foundation of China (30430570).

References

  1. 1.
    Abadulla E, Tzanov T, Costa S, Robra KH, Cavaco PA, Gubitz G (2000) Decolorization and detoxification of textile dyes with a laccase from Trametes hirsuta. Appl Environ Microbiol 66:3357–3362CrossRefGoogle Scholar
  2. 2.
    Alexandre G, Zhulin IB (2000) Laccase are widespread in bacteria. Trends Biotechnol 18:41–42CrossRefGoogle Scholar
  3. 3.
    Anthony V, Anissa LH, Durand SR (2004) Degradation of benzo[a]pyrene by mitosporic fungi and extracellular oxidative enzymes. Int Biodeterior Biodegradation 53:65–70CrossRefGoogle Scholar
  4. 4.
    Ardon O, Kerem Z, Hadar Y (1996) Enhancement of laccase activity in liquid cultures of the ligninolytic fungus Pleurotus ostreatus by cotton stalk extract. J Biotechnol 51:201–207CrossRefGoogle Scholar
  5. 5.
    Arora DS, Gill PK (2001) Effects of various media and supplements on laccase production by some white rot fungi. Bioresource Technol 77:89–91CrossRefGoogle Scholar
  6. 6.
    Baminger U, Subramaniam SS, Renganathan V, Haltrich D (2001) Purification and characterization of cellobiose dehydrogenase from the plant pathogen Sclerotium (Athelia) rolfsii. Appl Environ Microbiol 67:1766–1774CrossRefGoogle Scholar
  7. 7.
    Bollag JM, Leonowicz A (1984) Comparative studies of extracellular fungal laccases. Appl Environ Microbiol 48:849–854Google Scholar
  8. 8.
    Bourbonnails R, Paice MG (1997) Demethylation and delignification of kraft pulp by Trametes versicolor laccase in the presence of ABTS. Appl Environ Microbiol 36:823–827Google Scholar
  9. 9.
    Carmen R, Salas L, Vicuna R, Kirk T (1993) Extracellular enzyme production and synthetic lignin mineralization by Ceriporiopsis subvermispora. Appl Environ Microbiol 59:1792–1797Google Scholar
  10. 10.
    Cervantes C, Gutierrez-Corona F (1994) Copper resistance mechanisms in bacteria and fungi. FEMS Microbiol Rev 14:121–137CrossRefGoogle Scholar
  11. 11.
    Collins PJ, Dobson AD (1997) Regulation of laccase gene transcription in Trametes versicolor. Appl Environ Microbiol 63:3444–3450Google Scholar
  12. 12.
    Dekker RFH, Barbosa AM (2001) The effect of aeration and veratryl alcohol on the production of two laccases by the ascomycete Botryosphaeria sp. Enzyme Microb Technol 28:81–88CrossRefGoogle Scholar
  13. 13.
    Dittmer JK, Patel NJ, Dhawale SW, Dhawale SS (1997) Production of multiple laccase isoforms by Phanerochaete chrysosporium grown under nutrient sufficiency. FEMS Microbiol Lett 49:65–70CrossRefGoogle Scholar
  14. 14.
    Eggert C, Temp U, Eriksson K-EL (1996) The ligninolytic system of the white-rot fungus Pycnoporus cinnabarinus: purification and characterization of the laccase. Appl Environ Microbiol 62:1151–1158Google Scholar
  15. 15.
    Eggert C, Temp U, Eriksson K-EL (1996) Laccase-producing white-rot fungus lacking lignin peroxidase, and manganese peroxidase. ACS Symp Ser 655:130–150CrossRefGoogle Scholar
  16. 16.
    Fernandez-Larrea J, Stahl U (1996) Isolation and characterization of a laccase gene from Podospora anserina. Mol Gen Genet 252:539–551Google Scholar
  17. 17.
    Gianfreda L, Xu F, Bollag JM (1999) Laccases: a useful group of oxidoreductive enzymes. Bioremediation J 3:1–25CrossRefGoogle Scholar
  18. 18.
    Gigi O, Marbach I, Mayer AM (1891) Properties of gallic acid-induced extracellular laccase activity of Botrytis cinerea. Phytochemistry 20:1211–1213CrossRefGoogle Scholar
  19. 19.
    Hao J, Tian X, Song F, Zhang Z, Zhang P (2006) Involvement of lignocellulolytic enzymes in the decomposition of leaf litter in a subtropical forest. J Eukaryot Microbiol 53(3):193–198CrossRefGoogle Scholar
  20. 20.
    Hou H, Zhou J, Wang J, Du C, Yan B (2004) Enhancement of laccase production by Pleurotus ostreatus and its use for the decolorization of anthraquinone dye. Process Biochem 39:1415–1419CrossRefGoogle Scholar
  21. 21.
    Labbé S, Thiele DJ (1999) Pipes and wiring: the regulation of copper uptake and distribution in yeast. Trends Microbiol 7:500–505CrossRefGoogle Scholar
  22. 22.
    Lee I, Jung K, Lee C, Park Y (1999) Enhanced production of laccase in Trametes versicolor by the addition of ethanol. Biotechnol Lett 21:965–968CrossRefGoogle Scholar
  23. 23.
    Mansur M, Suárez T, González AE (1998) Differential gene expression in the laccase gene family from basidiomycete I-62 (CECT 20197). Appl Environ Microbiol 64:771–774Google Scholar
  24. 24.
    Niku-Paavola ML, Raaska L, Itavaara M (1990) Detection of white-rot fungi by a non-toxic stain. Mycol Res 94:27–31CrossRefGoogle Scholar
  25. 25.
    Nyanhongo GS, Gomes J, Gubitz GM, Zvauya R, Read JS, Steiner W (2002) Decolorization of textile dyes by laccases from a newly isolated strain of Trametes modesta. Water Res 36:1449–1456CrossRefGoogle Scholar
  26. 26.
    Nyanhongo G, Gomes J, Cubitz G, Zvauya R, Read J, Steiner W (2002) Production of laccase by a newly isolated strain of Trametes modesta. Bioresource Technol 84:259–263CrossRefGoogle Scholar
  27. 27.
    Palmieri G, Giardina P, Bianco C, Fontanella B, Sannia G (2000) Copper induction of laccase isoenzymes in the ligninolytic fungus Pleurotus ostreatus. Appl Environ Microbiol 66:920–924CrossRefGoogle Scholar
  28. 28.
    Pointing SB, Jones EBG, Vrijmoed LLP (2000) Optimization of laccase production by Pycnoporus sanguineus in submerged liquid culture. Mycologia 92:139–144Google Scholar
  29. 29.
    Robles A, Lucas R, Magdalena MC, Omar NB, Perez R, Antonio G (2002) Characterization of laccase activity produced by the hyphomycete Chalara (syn. Thielaviopsis) paradoxa CH32. Enzyme Microb Technol 31:516–522CrossRefGoogle Scholar
  30. 30.
    Ronne H (1995) Glucose repression in fungi. Trends Genet 11:12–17CrossRefGoogle Scholar
  31. 31.
    Savoie J, Mata G, Billette C (1998) Extracellular laccase production during hyphal interactions between Trichoderma sp. and Shiitake, Lentinula edodes. Appl Microbiol Biotechnol 49:589–593CrossRefGoogle Scholar
  32. 32.
    Sethuraman A, Akin DE, Erriksson KE (1999) Production of ligninolytic enzymes and synthetic lignin mineralization by the bird’s nest fungus Cyathus stercoreus. Appl Mcirobiol Biotechnol 52:689–697CrossRefGoogle Scholar
  33. 33.
    Thurston CF (1994) The structure and function of fungal laccases. Microbiology 140:19–26CrossRefGoogle Scholar
  34. 34.
    Xu F (1999) Laccase. In: Flickinger MC, Drew SW (eds) Encyclopedia of bioprocess technology: fermentation, biocatalysis, and bioseparation, vol 3. Wiley, New York, pp 1545–1554Google Scholar
  35. 35.
    Zhang L, Zhang T, Li L (1981) Experimental methods and technology of biochemistry. Higher Education Press, Beijing, pp 372–373Google Scholar

Copyright information

© Society for Industrial Microbiology 2006

Authors and Affiliations

  • Jiejie Hao
    • 1
  • Fuqiang Song
    • 1
    • 2
  • Feng Huang
    • 1
  • Changlin Yang
    • 1
  • Zhijun Zhang
    • 1
    • 2
  • Yi Zheng
    • 1
  • Xingjun Tian
    • 1
    Email author
  1. 1.School of Life ScienceNanjing UniversityNanjingChina
  2. 2.Key Laboratory for Modern Sylvicultural Technology of Zhejiang ProvinceZhejiang Forestry CollegeZhejiangChina

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