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Ammonia removal from livestock wastewater by ammonia-assimilating microorganisms immobilized in polyvinyl alcohol

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Journal of Industrial Microbiology & Biotechnology

Abstract

We isolated ammonia-assimilating microorganisms from the livestock manure treatment systems and evaluated their ammonia-assimilating ability. Many isolates utilized ammonia at high rates when they were purely cultivated in a nitrogen-limited medium to which sterilized lagoon extract had been added. Some isolates that were immobilized in polyvinyl alcohol (PVA) utilized ammonia present in the media containing viable lagoon microorganisms. Staining with 4′,6′-diamidino-2-phenylindole (DAPI) indicated that the immobilized high ammonia-assimilating isolates grew dominantly within the PVA beads. High ammonia-assimilating isolates in the mixed culture containing viable lagoon microorganisms were identified as Pseudomonas spp. and member of Rhizobiaceae species by partial sequencing of the 16S ribosomal DNA.

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Acknowledgment

This study was supported in part by a grant from the Livestock Technology Association, Japan.

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Authors and Affiliations

  1. Laboratory of Animal Health and Management, Graduate School of Agricultural Science, Tohoku University, Naruko, Miyagi, 989-6711, Japan

    Hiraku Sasaki, Jun Nonaka, Takako Sasaki & Yutaka Nakai

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  1. Hiraku Sasaki
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  2. Jun Nonaka
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  3. Takako Sasaki
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  4. Yutaka Nakai
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Correspondence to Yutaka Nakai.

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Sasaki, H., Nonaka, J., Sasaki, T. et al. Ammonia removal from livestock wastewater by ammonia-assimilating microorganisms immobilized in polyvinyl alcohol. J Ind Microbiol Biotechnol 34, 105–110 (2007). https://doi.org/10.1007/s10295-006-0172-6

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  • DOI: https://doi.org/10.1007/s10295-006-0172-6

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