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Novel organization of catechol meta pathway genes in the nitrobenzene degrader Comamonas sp. JS765 and its evolutionary implication

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  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

The catechol meta cleavage pathway is one of the central metabolic pathways for the degradation of aromatic compounds. A novel organization of the pathway genes, different from that of classical soil microorganisms, has been observed in Sphingomonas sp HV3 and Pseudomonas sp. DJ77. In a Comamonas sp. JS765, cdoE encoding catechol 2,3-dioxygenase shares a common ancestry only with tdnC of a Pseudomonas putida strain, while codG encoding 2-hydroxymuconic semialdehyde dehydrogenase shows a higher degree of similarity to those genes in classical bacteria. Located between cdoE and cdoG are several putative genes, whose functions are unknown. These genes are not found in meta pathway operons of other microorganisms with the exception of cdoX2, which is similar to cmpX in strain HV3. Therefore, the gene cluster in JS765 reveals a third type of gene organization of the meta pathway.

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Abbreviations

C23O:

Catechol 2,3-dioxygenase

HMSD-2:

Hydroxymuconic semialdehyde dehydrogenase

HMSH-2:

Hydroxymuconic semialdehyde hydrolase

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Acknowledgments

Z. He thank Heather Wright for her assistance in figure preparation. Trade or manufacturers’ names mentioned in the paper are for information only and do not constitute endorsement, recommendation, or exclusion by the USDA-ARS.

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Authors and Affiliations

  1. USDA-ARS, New England Plant, Soil, and Water Laboratory, Orono, ME, 04469, USA

    Zhongqi He

  2. Section of Microbiology, University of California, Davis, CA, 95616, USA

    Rebecca E. Parales

  3. Environmental Engineering Program, Georgia Institute of Technology, Atlanta, GA, 30332, USA

    Jim C. Spain

  4. Air Force Research Laboratory, Tyndall Air Force Base, FL, 32403, USA

    Glenn R. Johnson

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  1. Zhongqi He
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  2. Rebecca E. Parales
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Correspondence to Zhongqi He.

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He, Z., Parales, R.E., Spain, J.C. et al. Novel organization of catechol meta pathway genes in the nitrobenzene degrader Comamonas sp. JS765 and its evolutionary implication. J Ind Microbiol Biotechnol 34, 99–104 (2007). https://doi.org/10.1007/s10295-006-0170-8

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  • DOI: https://doi.org/10.1007/s10295-006-0170-8

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