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Effect of salt nutrients on mannitol production by Lactobacillus intermedius NRRL B-3693

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Journal of Industrial Microbiology and Biotechnology


The effects of four salt nutrients (ammonium citrate, sodium phosphate, magnesium sulfate, and manganese sulfate) on the production of mannitol by Lactobacillus intermedius NRRL B-3693 in a simplified medium containing 300 g fructose, 5 g soy peptone, and 50 g corn steep liquor per liter in pH-controlled fermentation at 5.0 at 37°C were evaluated using a fractional factorial design. Only manganese sulfate was found to be essential for mannitol production. Added manganese sulfate concentration of 0.033 g/l was found to support maximum production. The bacterium produced 200.6±0.2 g mannitol, 61.9±0.1 g lactic acid, and 40.4±0.3 g acetic acid from 300 g fructose per liter in 67 h.

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The author thanks Gregory J. Kennedy for excellent technical assistance.

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Correspondence to Badal C. Saha.

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Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture.

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Saha, B.C. Effect of salt nutrients on mannitol production by Lactobacillus intermedius NRRL B-3693 . J IND MICROBIOL BIOTECHNOL 33, 887–890 (2006).

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