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Influence of high salinity on biofilm formation and benzoate assimilation by Pseudomonas aeruginosa

  • Original Paper
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Journal of Industrial Microbiology & Biotechnology

Abstract

Pseudomonas species were used in bioremediation technologies. In situ conditions, such as marine salinity, could limit the degradation of hydrocarbons and aromatic compounds by the bacteria. Biofilm ability to tolerate environmental stress could be used to increase biorestoration. In this report, we used scanning confocal laser microscopy and microtiter dish assay to analyse the impact of hyperosmotic stress on biofilm formation by Pseudomonas aeruginosa. We used benzoate as the sole carbon source and the effect of the stress on its degradation was also studied. Hyperosmotic shock inhibited the biofilm development and decreased the degradation of benzoate. The osmoprotectant glycine betaine partially restored both the biofilm formation and benzoate degradation, suggesting that it could be used as a complement in bioremediation processes.

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Acknowledgements

This work was supported by the Region Bretagne, FEDER funds, and the Ministère de la Recherche et de la Technologie (RITMER grant and doctoral fellowships to A.B. and F. D.). We thank G. A. O’Toole for the kind gift of plasmid pSMC21. We thank A. Dufour for his critically reading manuscript.

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Authors and Affiliations

  1. Laboratoire de Biotechnologie et Chimie Marines, EA 3884, Université de Bretagne Sud, BP 92116, 56321, Lorient Cédex, France

    Alexis Bazire & Dominique Haras

  2. Département Osmorégulation chez les Bactéries, UMR-CNRS 6026, Université de Rennes I, Rennes, France

    Farès Diab & Mohamed Jebbar

Authors
  1. Alexis Bazire
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  2. Farès Diab
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  3. Mohamed Jebbar
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  4. Dominique Haras
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Correspondence to Alexis Bazire.

Additional information

Alexis Bazire and Farès Diab contributed equally to this work.

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Bazire, A., Diab, F., Jebbar, M. et al. Influence of high salinity on biofilm formation and benzoate assimilation by Pseudomonas aeruginosa . J Ind Microbiol Biotechnol 34, 5–8 (2007). https://doi.org/10.1007/s10295-006-0087-2

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  • DOI: https://doi.org/10.1007/s10295-006-0087-2

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