Abstract
In this study, the effects of inositol addition on expression of the MAL gene encoding maltase and phosphatidylinositol (PI) biosynthesis in Schizosaccharomyces pombe (a naturally inositol-requiring strain) were examined. We found that specific maltase activity was at its maximum when the concentration of added inositol reached 6 μg ml−1 in a synthetic medium containing 2.0% (w/v) glucose. When the concentration of added inositol was 1 μg ml−1 in the medium, repression of MAL gene expression occurred at glucose concentration higher than 0.2% (w/v). However, when S. pombe was cultured in the synthetic medium containing 6 μg ml−1, repression of maltase gene expression occurred only at initial glucose concentration above 1.0% (w/v). More mRNA encoding maltase was detected in the cells grown in the medium with 6 μg ml−1 inositol than in those grown in the same medium with 1 μg ml−1 inositol. These results demonstrate that higher inositol concentrations in the synthetic medium could derepress MAL gene expression in S. pombe. PI content of the yeast cells grown in the synthetic medium with 6 μg ml−1 of inositol was higher than that of the yeast cells grown in the same medium with 1 μg ml−1 of inositol. This means that PI may be involved in the derepression of MAL gene expression in S. pombe.
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Abbreviations
- PC:
-
Phosphatidylcholine
- PE:
-
Phosphatidylethanolamine
- PA:
-
Phosphatidyl acid
- PI:
-
Phosphatidylinositol
- PS:
-
Phosphatidylserine
- CL:
-
Cardiolipin
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The authors thank the National Natural Science Foundation of China for its financial support. The Grant no is 30370015.
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Yao, S., Chi, Z. & He, S. Studies on inositol-mediated expression of MAL gene encoding maltase and phospholipid biosynthesis in Schizosaccharomyces pombe . J IND MICROBIOL BIOTECHNOL 33, 417–422 (2006). https://doi.org/10.1007/s10295-005-0069-9
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DOI: https://doi.org/10.1007/s10295-005-0069-9