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A wide-range integrative yeast expression vector system based on Arxula adeninivorans-derived elements

  • Original Paper
  • Published:
Journal of Industrial Microbiology and Biotechnology

Abstract

An Arxula adeninivorans integration vector was applied to a range of alternative yeast species including Saccharomyces cerevisiae, Debaryomyces hansenii, Debaryomyces polymorphus, Hansenula polymorpha and Pichia pastoris. The vector harbours a conserved A. adeninivorans-derived 25S rDNA sequence for targeting, the A. adeninivorans-derived TEF1 promoter for expression control of the reporter sequence, and the Escherichia coli-derived hph gene conferring resistance against hygromycin B for selection of recombinants. Heterologous gene expression was assessed using a green fluorescent protein (GFP) reporter gene. The plasmid was found to be integrated into the genome of the various hosts tested; recombinant strains of all species exhibited heterologous gene expressions of a similar high level.

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Acknowledgements

We are grateful to Dr. I. Kunze for helpful discussion and critical reading of the manuscript. We also thank H. Bohlmann and R. Franz for excellent technical assistance. The experimental work was supported by grants from the Ministry of Economic, Nordrhein-Westfalen (TPW-9910v08), Bundesstiftung Umwelt (AZ 13048) and by Funds of the Chemical Industry (to G.K.).

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Author notes

  1. Gerd Gellissen

    Present address: Med Artis Pharmacenticals, Pauwelstraße 19, 52047, Aachen, Germany

Authors and Affiliations

  1. Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstrasse 3, 06466, Gatersleben, Germany

    Yaroslav Terentiev, Almudena Huarto Pico, Erik Böer, Thomas Wartmann & Gotthard Kunze

  2. Institut für Mikrobiologie, Heinrich-Heine-Universität Düsseldorf, Universitätsstrasse 1, 40225, Düsseldorf, Germany

    Jens Klabunde

  3. Umweltforschungszentrum Leipzig-Halle (UFZ), Permoserstrasse 15, 04318, Leipzig, Germany

    Uta Breuer & Wolfgang Babel

  4. Rhein Biotech, Eichsfelder Strasse 11, 40595, Düsseldorf, Germany

    Manfred Suckow & Gerd Gellissen

Authors
  1. Yaroslav Terentiev
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  2. Almudena Huarto Pico
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  10. Gotthard Kunze
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Correspondence to Gotthard Kunze.

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Terentiev, Y., Pico, A.H., Böer, E. et al. A wide-range integrative yeast expression vector system based on Arxula adeninivorans-derived elements. J IND MICROBIOL BIOTECHNOL 31, 223–228 (2004). https://doi.org/10.1007/s10295-004-0142-9

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