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Glutamate as an inhibitor of phosphoenolpyruvate carboxylase activity in Corynebacterium glutamicum

  • Original Paper
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Journal of Industrial Microbiology and Biotechnology

Abstract

The glutamate-producing bacterium, Corynebacterium glutamicum is known to possess two anaplerotic enzymes: pyruvate carboxylase (Pc) and phosphoenolpyruvate carboxylase (PEPc). In vitro, this latter enzyme appeared to be inhibited by different glutamic acid salts, whereas ammonium-glutamate had no influence on Pc activity. To investigate the in vivo relevance of PEPc activity inhibition, the intracellular concentration of glutamate was determined throughout the glutamate-producing process. The intracellular concentration was then shown to be sufficient to induce a dramatic inhibition of PEPc activity during the process. As a consequence, intracellular accumulation of glutamate could be at least partially responsible for the weak participation of PEPc within the anaplerosis activity in amino-acid-producing strains of C. glutamicum.

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Acknowledgements

This work was supported by the CNRS (GdR 1157 and 1597 and Programme Interdisciplinaire de Recherche en Génie des Procédés).

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Author notes

  1. Pascale Daran-Lapujade

    Present address: Kluyver Laboratory of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands

Authors and Affiliations

  1. Laboratoire des Sciences du Génie Chimique (CNRS), Institut National Polytechnique de Lorraine, 2 Avenue de la Forêt de Haye, BP 172, 54505, Vandoeuvre-lès-Nancy, France

    Stéphane Delaunay, Pascale Daran-Lapujade & Jean-Louis Goergen

  2. Laboratoire Bioprocédés Agroalimentaires, Institut National Polytechnique de Lorraine, 2 Avenue de la Forêt de Haye, BP 172, 54505, Vandoeuvre-lès-Nancy Cedex, France

    Jean-Marc Engasser

Authors
  1. Stéphane Delaunay
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  2. Pascale Daran-Lapujade
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  3. Jean-Marc Engasser
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  4. Jean-Louis Goergen
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Correspondence to Stéphane Delaunay.

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Delaunay, S., Daran-Lapujade, P., Engasser, JM. et al. Glutamate as an inhibitor of phosphoenolpyruvate carboxylase activity in Corynebacterium glutamicum . J IND MICROBIOL BIOTECHNOL 31, 183–188 (2004). https://doi.org/10.1007/s10295-004-0137-6

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  • DOI: https://doi.org/10.1007/s10295-004-0137-6

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