Abstract
Growth and production of lipase by a new Geotrichum-like strain, R59, were studied. Production of extracellular lipase was substantially enhanced when the initial pH of the culture medium, types of carbon and nitrogen sources, substances probably stimulating the lipase biosynthesis, the temperature, and time of growth were optimized. Sucrose and triolein were the most effective carbon sources for lipase production. Maximum lipase activity (146 U/ml−1) was obtained with urea as the nitrogen source. Growth at 30°C, an initial pH of 6.0 and incubation time of 48 h were found as optimum conditions for cell growth and production of lipase by Geotrichum-like strain R59. The enzyme was thermostable and exhibited very high activity after 1 h incubation at 60°C.
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References
Baillargeon MW, Bistline RG, Sonnet PE (1989) Evaluation of strains of Geotrichum candidum for lipase production and fatty acid specificity. Appl Microbiol Biotechnol 30:92–96
Cardenas J, Alvarez E, de Castro-Alvarez MS, Sanchez-Montero JM, Valmaseda M, Elson SW, Sinisterra JV (2001) Screening and catalytic activity in organic synthesis of novel fungal and yeast lipases. J Mol Catal B 14:111–123
Chander H, Sannabhadti SS, Elias J, Ranganathan B (1977) Factors affecting lipase production by Penicillium chrysogenum. J Food Sci 42:1677–1678
Corzo R, Revah S (1999) Production and characteristics of the lipase from Yarrowia lipolytica 681. Bioresour Technol 70:173–180
Costa MAP, Peralta RM (1999) Production of lipase by soil fungi and partial characterization of lipase from a selected strain (Penicillium wortmanii). J Basic Microbiol 39:11–15
Dean JA (1978) Lange’s handbook of chemistry, 12th edn. McGraw-Hill, New York, pp 5–79
Domsch KH, Gams W, Anderson TH (1980) Compendium of soil fungi, vol 1. Academic, London
Fodiloglu S, Erkmen O (1999) Lipase production by Rhizopus oryzae growing on different carbon and nitrogen sources. J Sci Food Agric 79:1936–1938
Ghanem EH, Al-Sayed HA, Saleh KM (2000) An alkalophilic thermostable lipase produced by a new isolate of Bacillus alcalophilus. World J Microbiol Biotechnol 16:459–464
Ghosh PK, Saxena TK, Gupta R, Yadav RP, Davidson S (1996) Microbial lipases: production and applications. Sci Prog 79:119–157
Kamini NR, Fujii T, Kurosu T, Iefuji H (2000) Production, purification and characterization of an extracellular lipase from the yeast, Cryptococcus sp. S-2. Process Biochem 36:317–324
Kulkarni N, Gadre RV (2002) Production and properties of an alkaline, thermophilic lipase from Pseudomonas fluorescens NS2 W. J Ind Microbiol Biotechnol 28:344–348
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Macedo GA, Park YK, Pastore GM (1997) Partial purification and characterization of an extracellular lipase from a newly isolated strain of Geotrichum sp. Rev Microbiol 28:90–95
Mates A, Sudakevitz D (1973) Production of lipase by Staphylococcus aureus. J Appl Bacteriol 36:219
Nawani N, Dosanjh NS, Kaur J (1998) A novel thermostable lipase from a thermophilic Bacillus sp.: characterization and esterification studies. Biotechnol Lett 20:997–1000
Obradors N, Montesinos JL, Valero F, Lafuente FJ, Sola C (1993) Effect of different fatty acids in lipase production by Candida rugosa. Biotechnol Lett 15:357–360
Ota Y, Miyairi S, Yamada K (1968) Sterol requirement for the lipase production by Candida cylindracea. Agric Biol Chem 32:1476–1478
Papaparaskevas D, Christakopoulos P, Kekos D, Macris BJ (1992) Optimizing production of extracellular lipase from Rhodotorula glutinis. Biotechnol Lett 14:397–402
Salleh AB, Musani R, Razak CNA (1993) Extra and intracellular lipases from a thermophilic Rhizopus oryzae and factors affecting their production. Can J Microbiol 39:978–981
Sarkar S, Sreekanth B, Kant S, Banerjee R, Bhattacharyya BC (1998) Production and optimization of microbial lipase. Bioprocess Eng 19:29–32
Shimada Y, Sugihara Y, Nagae T, Tominaga Y (1992) Induction of Geotrichum candidum lipase by long-chain fatty acids. J Ferment Bioeng 74:77–80
Sokolovska I, Albasi C, Riba JP, Bales V (1998) Production of extracellular lipase by Candida cylindracea CBS 6330. Bioprocess Eng 19:179–186
Sommer P, Bormann C, Gotz F (1997) Genetic and biochemical characterization of a new extracellular lipase from Streptomyces cinnamomeus. Appl Environ Microbiol 63:3553–3560
Sugihara A, Shimada Y, Tominaga Y (1990) Separation and characterization of two molecular forms of Geotrichum candidum lipase. J Biochem 107:426–430
Wang Y, Srivastava KC, Shen GJ, Wang HY (1995) Thermostable alkaline lipase from a newly isolated thermophilic Bacillus strain, A30-1 (ATCC 53841). J Ferment Bioeng 79:433–438
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This work was supported by the Polish Science Committee BS/BiNoZ/4.
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Ginalska, G., Bancerz, R. & Korniłłowicz-Kowalska, T. A thermostable lipase produced by a newly isolated Geotrichum-like strain, R59. J IND MICROBIOL BIOTECHNOL 31, 177–182 (2004). https://doi.org/10.1007/s10295-004-0134-9
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DOI: https://doi.org/10.1007/s10295-004-0134-9