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Antifungal activity of Bacillus subtilis 355 against wood-surface contaminant fungi

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Journal of Industrial Microbiology and Biotechnology

Abstract

A strain of Bacillus subtilis was examined for antifungal activity against phytopathogenic and wood-surface contaminant fungi. The bacterium was grown in five culture media with different incubation times in order to study cell development, sporulation, and the production of metabolites with antifungal activity. The anti-sapstain and anti-mould activity of the bacterium grown in yeast extract glucose broth (YGB) medium in wood was also evaluated. In YGB, the bacterium inhibited the growth of several fungi and displayed a broader spectrum of activity than in the other media tested. A relationship between bacterial spore production and the formation of metabolites with antifungal activity was detected. YGB medium displayed effective control in wood block tests. YGB medium was extracted with solvents of increasing polarity and the dry residues were applied to silicagel plates, resolved with the appropriate solvent and sprayed with different solutions, detecting the presence, of amines, and higher alcohols. The bioautographic method revealed the presence of at least two active compounds against the blue-stain fungus Cladosporium cucumerinum.

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Acknowledgements

This work was supported by Fundação de Ciência e Tecnologia and by FEDER. Project 391647BSE/2001.

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Authors and Affiliations

  1. Departamento de Tecnologia de Indústrias Químicas, Instituto Nacional de Engenharia e Tecnologia Industrial, Estrada ao Paço do Lumiar, 1699-038, Lisbon, Portugal

    Sonia Savluchinske Feio, Ana Barbosa, Manuela Cabrita, Alexandra Esteves, José Carlos Roseiro & Maria João Marcelo Curto

  2. Laboratório Nacional de Engenharia Civil, Av. do Brasil, 101, 1700-066, Lisbon, Portugal

    Lina Nunes

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  1. Sonia Savluchinske Feio
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  2. Ana Barbosa
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  3. Manuela Cabrita
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  4. Lina Nunes
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  5. Alexandra Esteves
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  6. José Carlos Roseiro
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  7. Maria João Marcelo Curto
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Correspondence to Sonia Savluchinske Feio.

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Feio, S.S., Barbosa, A., Cabrita, M. et al. Antifungal activity of Bacillus subtilis 355 against wood-surface contaminant fungi. J IND MICROBIOL BIOTECHNOL 31, 199–203 (2004). https://doi.org/10.1007/s10295-004-0133-x

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  • DOI: https://doi.org/10.1007/s10295-004-0133-x

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