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Microbial characterisation of activated sludge in jet-loop bioreactors treating winery wastewaters

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Journal of Industrial Microbiology and Biotechnology

Abstract

Jet-loop reactors (JLR) used as biological waste treatment processes introduce an additional selective pressure on the natural microbial flora of the incoming effluent. Several high-performing microbial inocula were tested for winery wastewater treatment and the microbial composition was analysed. A microbial consortium was enriched and selected for use with a new type of aerobic JLR. The reactor was operated continuously for more than 1 year using winery wastewaters collected in different seasons. Chemical oxygen demand (COD) removal efficiency was on average greater than 80%, with retention times of 0.8–1 day. Microbial populations were sampled for characterisation after 6 months and at the end of the study. Isolates were identified at genus and/or species level. Almost all isolates belonged to the genera Pseudomonas and Bacillus. Saccharomyces cerevisiae was also found but no filamentous fungi. These results show that a highly adapted population develops in JLRs treating winery effluents as compared to other bioreactors. Aerobic JLRs impose a stringent selective criterion on the composition of the microbial biomass.

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Acknowledgements

The authors thank Elsa Almeida-Vara and Gabriela Sena-Martins, at INETI, for review of the manuscript and their useful comments. The European Commission, DG Environment, within the Programme LIFE (Project 96ENV/P/00602/INDW), sponsored this research work.

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Authors and Affiliations

  1. Biotechnology Department, INETI, Estrada do Paço do Lumiar 22, 1649-038 , Lisbon, Portugal

    Ana Eusébio, Manuela Lageiro & José Cardoso Duarte

  2. Dipartimento di Agrobiologia e Agrochimica, University of Tuscia, Via San Camillo De Lellis 1, 01100 , Viterbo, Italy

    Maurizio Petruccioli & Federico Federici

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  1. Ana Eusébio
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  2. Maurizio Petruccioli
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  3. Manuela Lageiro
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  4. Federico Federici
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  5. José Cardoso Duarte
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Correspondence to José Cardoso Duarte.

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Eusébio, A., Petruccioli, M., Lageiro, M. et al. Microbial characterisation of activated sludge in jet-loop bioreactors treating winery wastewaters. J IND MICROBIOL BIOTECHNOL 31, 29–34 (2004). https://doi.org/10.1007/s10295-004-0111-3

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  • DOI: https://doi.org/10.1007/s10295-004-0111-3

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