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Sequences affecting the regulation of solvent production in Clostridium acetobutylicum

  • Original Paper
  • Published:
Journal of Industrial Microbiology and Biotechnology

Abstract

The high solvent phenotype of Clostridium acetobutylicum mutants B and H was complemented by the introduction of a plasmid that contains either an intact or partially-deleted copy of solR, restoring acetone and butanol production to wild-type levels. This demonstrates that the solR open reading frame on pSOLThi is not required to restore solvent levels. The promoter region upstream of alcohol dehydrogense E (adhE) was examined in efforts to identify sites that play major roles in the control of expression. A series of adhE promoter fragments was constructed and the expression of each in acid- and solvent-phases of growth was analyzed using a chloramphenicol acetyl-transferase reporter system. Our results show that a region beyond the 0A box is needed for full induction of the promoter. Additionally, we show that the presence of sequences around a possible processing site designated S2 may have a negative role in the regulation of adhE expression.

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Acknowledgements

This research was supported by the National Science Foundation BES-0001288, and by Robert A. Welch Foundation grants C-1268 and C-1372.

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  1. Ke-xue Huang

    Present address: Dow Agrosciences LLC, R & D Discovery, 9330 Zionsville Road, Indianapolis, IN 46268, USA

Authors and Affiliations

  1. Department of Biochemistry and Cell Biology, MS 140, Rice University, 6100 Main Street, Houston, TX 77005, USA

    Miles C. Scotcher, Ke-xue Huang, Mary L. Harrison, Frederick B. Rudolph & George N. Bennett

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  1. Miles C. Scotcher
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  2. Ke-xue Huang
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  3. Mary L. Harrison
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  4. Frederick B. Rudolph
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  5. George N. Bennett
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Correspondence to George N. Bennett.

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Scotcher, M.C., Huang, Kx., Harrison, M.L. et al. Sequences affecting the regulation of solvent production in Clostridium acetobutylicum . J IND MICROBIOL BIOTECHNOL 30, 414–420 (2003). https://doi.org/10.1007/s10295-003-0057-x

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  • DOI: https://doi.org/10.1007/s10295-003-0057-x

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