Abstract
An efficient screening method following UV mutagenesis yielded a high frequency of improved mutants of Trichosporon brassicae CGMCC 0574, a wild-type esterase-producer capable of enantioselectively hydrolyzing the ethyl ester of ketoprofen [2-(3-benzoylphenyl) propionic acid]. The mutant had an activity 1.8-fold higher than the wild type and was stable in its enzyme production for ten serial transfers. As the best single carbon source, isopropanol improved the specific activity of the enzyme 5-fold; and this did not result from the effect of cell permeabilization. An 18-h culture grown on a medium containing 0.5% glucose plus 0.5% isopropanol produced 3-fold as much esterase as a culture grown on 1% glucose.
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Acknowledgements
Financial support from the National Natural Science Foundation of China (grant 20176011) and the Shanghai Commission of Science and Technology (grant 98QB14042) is gratefully acknowledged. We also thank Professor Qin Ye at our State Key Laboratory of Bioreacotor Engineering for helpful discussions and suggestions in the improvement of this paper.
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Wu, HY., Xu, JH., Shen, D. et al. Improved production of (S)-ketoprofen ester hydrolase by a mutant of Trichosporon brassicae CGMCC 0574. J IND MICROBIOL BIOTECHNOL 30, 357–361 (2003). https://doi.org/10.1007/s10295-003-0053-1
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DOI: https://doi.org/10.1007/s10295-003-0053-1