Abstract
Cytochromes are membrane-bound hemoproteins responsible for the generation of ATP via the electron transport system to fuel the metabolic processes of the organism for their growth. This study reports the properties of cytochromes present in the isolated lichenized fungi of the cultured lichen Usnea ghattensis under optimized conditions. The fungal partner of the cultured lichen Usnea ghattensis contains a, b and c types of cytochromes. The concentrations of a, b and c type cytochromes were found to be significantly high (0.0967, 0.0900, and 0.1030 mM/mg protein, respectively) in the isolated fungal symbiont of cultured lichen grown in malt-yeast extract medium supplemented with 0.01 mol/l sucrose and 0.01 mol/l polyethylglycol. The results suggest that supplementation of additional carbon sources may play a role in optimizing the growth via activating the cytochrome respiratory system in lichenized fungi.
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Acknowledgments
We are very grateful to the Director of Agharkar Research Institute, Pune, India, for providing research facilities and financial support by the Department of Biotechnology (DBT) (grant no. BT/PR 3133/BCE/08/237/2002), Council of Scientific and Industrial Research (CSIR) India (grant no. 9/670(034)/2006-EMR-I).
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Verma, N., Behera, B.C. & Makhija, U.V. Studies on cytochromes of lichenized fungi under optimized culture conditions. Mycoscience 52, 65–68 (2011). https://doi.org/10.1007/s10267-010-0070-7
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DOI: https://doi.org/10.1007/s10267-010-0070-7