Abstract
In this study, two plots in a secondary and another two in planted Pinus densiflora stands were exposed to different forest treatments, and the ectomycorrhizal (EM) biomass and its ergosterol content was measured for a year. The unmanaged plot in the secondary stand had greater EM biomass than those in any other plots. Whereas understory cutting had less effect on EM biomass, litter and humus removal decreased pine EM biomass and its ergosterol content, suggesting that such forest treatment alters EM biomass and its structure.
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References
Andrews JA, Harrison KG, Matamala R, Schlesinger WH (1999) Separation of root respiration from total soil respiration using carbon-13 labeling during free-air carbon dioxide enrichment (FACE). Soil Sci Soc Am J 63:1429–1435
Antibus RK, Sinsabaugh RL (1993) The extraction and quantification of ergosterol from ectomycorrhizal roots. Mycorrhiza 3:137–144
Baar J, Kuyper TW (1993) Litter removal in forests and effect on mycorrhizal fungi. In: Pegler DN, Boddy L, Ing B, Kirk PM (eds) Fungi of Europe: investigation, recording and conservation. Royal Botanic Gardens, Kew, pp 275–286
Baar J, Kuyper TW (1998) Restoration of aboveground ectomycorrhizal flora in stands of Pinus sylvestris (Scots pine) in The Netherlands by removal of litter and humus. Rest Ecol 6:227–237
Baar J, Ter Braak CJF (1996) Ectomycorrhizal sporocarp occurrence as affected by manipulation of litter and humus layers in Scots pine stands of different age. Appl Soil Ecol 4:61–73
Dahlberg A, Jonsson L, Nylund JE (1997) Species diversity and distribution of biomass above and below ground among ectomycorrhizal fungi in an old-growth Norway spruce forest in south Sweden. Can J Bot 75:1323–1335
Helmisaari HS, Makkonen K, Kellomäki S, Valtonen E, Mälkönen E (2002) Below- and above-ground biomass, production and nitrogen use in Scots pine stands in eastern Finland. For Ecol Manag 165:317–326
Helmisaari HS, Ostonen I, Lõhmus K, Derome J, Lindroos AJ, Merilä P, Nöjd P (2009) Ectomycorrhizal root tips in relation to site and stand characteristics in Norway spruce and Scots pine stands in boreal forests. Tree Physiol 29:445–456
Hobbie EA (2006) Carbon allocation to ectomycorrhizal fungi correlates with belowground allocation in culture studies. Ecology 87:563–569
Högberg P, Nordgren A, Buchmann N, Taylor AFS, Ekblad A, Högberg MN, Nyberg G, Ottosson-Löfvenius M, Read DJ (2001) Large-scale forest girdling shows that current photosynthesis drives soil respiration. Nature 411:789–792
Ito T, Ogawa M (1979) Cultivating method of the mycorrhizal fungus, Tricholoma matsutake (Ito et Imai) Sing. (II): increasing numbers of Shiro (fungal colony) of T. matsutake by thinning the understory vegetation (in Japanese with English summary). J Jpn For Soc 61:163–173
Kake Y, Matsushita N, Suzuki K (2000) Effects of forest operations on ectomycorrhizal fungi in a Japanese red pine (Pinus densiflora) stand (in Japanese with English summary). Bul Tokyo Univ For 104:147–156
Kikuchi T, Aikawa T, Oeda Y, Karim N, Kanzaki N (2009) A rapid and precise diagnostic method for detecting the pinewood nematode Bursaphelenchus xylophilus by loop-mediated isothermal amplification. Phytopathology 99:1365–1369
Kume A, Satomura T, Tsuboi N, Chiwa M, Hanba YT, Nakane K, Horikoshi T, Sakugawa H (2003) Effects of understory vegetation on the ecophysiological characteristics of an overstory pine, Pinus densiflora. For Ecol Manag 176:195–203
Lian C, Narimatsu M, Nara K, Hogetsu T (2006) Tricholoma matsutake in a natural Pinus densiflora forest: correspondence between above- and below-ground genets, association with multiple host trees and alteration of existing ectomycorrhizal communities. New Phytol 171:825–836
Mamiya Y (1983) Pathology of the pine wilt disease caused by Bursaphelenchus xylophilus. Annu Rev Phytopathol 21:201–220
Murata H, Babasaki K, Saegusa T, Takemoto K, Yamada A, Ohta A (2008) Traceability of Asian Matsutake, specialty mushrooms produced by the ectomycorrhizal basidiomycete Ticholoma matsutake, on the basis of retroelement-based DNA markers. Appl Environ Microbiol 74:2023–2031
Nylund JE, Wallander H (1992) Ergosterol analysis as a means of quantifying mycorrhizal biomass. In: Norris JR, Read DJ, Varma AK (eds) Methods in microbiology 24: techniques for the study of mycorrhiza. Academic Press, London, pp 77–88
Persson HA (1983) The distribution and productivity of pine roots in boreal forests. Plant Soil 71:87–101
Richardson DM (ed) (1998) Ecology and biogeography of Pinus. Cambridge University Press, Cambridge
Salmanowicz B, Nylund JE (1988) High-performance liquid-chromatography determination of ergosterol as a measure of ectomycorrhiza infection in Scots pine. Eur J For Pathol 18:291–298
Satomura T, Nakatsubo T, Horikoshi T (2003) Estimation of the biomass of fine roots and mycorrhizal fungi: a case study in a Japanese red pine (Pinus densiflora) stand. J For Res 8:221–225
Sayer EJ (2006) Using experimental manipulation to assess the roles of leaf litter in the functioning of forest ecosystems. Biol Rev 81:1–31
Smith SE, Read DJ (2008) Mycorrhizal symbiosis, 3rd edn. Academic Press, London
Wallander H, Massicotte HB, Nylund JE (1997) Seasonal variation in protein, ergosterol and chitin in five morphotypes of Pinus sylvestris L. ectomycorrhizae in a mature Swedish forest. Soil Biol Biochem 29:45–53
Yamada A, Kanekawa S, Ohmasa M (1999) Ectomycorrhiza formation of Tricholoma matsutake on Pinus densiflora. Mycoscience 40:193–198
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This work was supported in part by Grant-in-Aid for Scientific Research No. 19380085 from the Ministry of Education, Culture, Sports, Science and Technology.
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Okada, K., Satomura, T., Kinoshita, A. et al. Difference of pine ectomycorrhizal biomass in relation to forest conditions. Mycoscience 52, 59–64 (2011). https://doi.org/10.1007/s10267-010-0069-0
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DOI: https://doi.org/10.1007/s10267-010-0069-0