Skip to main content
SpringerLink
Log in

A new acidophilic fungus Teratosphaeria acidotherma (Capnodiales, Ascomycota) from a hot spring

  • Full Paper
  • Published:
Mycoscience

Abstract

A novel acidophilic fungus was isolated by an acidic enrichment culture of microbial mats and biofilms collected at an extremely acidic and high temperature hot spring. In culture studies, this fungus was revealed to produce ascomycetous teleomorph structures. Molecular phylogenetic study and morphological observation showed this fungus is a new species of the genus Teratosphaeria (Capnodiales, Dothideomycetes) and is phylogenetically close to Acidomyces acidophilus and Bispora sp., which were previously reported as acidophilic anamorphic fungi. This new fungus is described here as a new species of Teratosphaeria, and its physiological properties adapting to its habitat are demonstrated. This is the first report of a teleomorphic fungus having highly acidophilic and thermophilic properties.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10

Similar content being viewed by others

References

  • Baker BJ, Banfield JF (2003) Microbial communities in acid mine drainage. FEMS Microbiol Ecol 44:139–152

    Article  CAS  PubMed  Google Scholar 

  • Baker BJ, Lutz MA, Dawson SC, Bond PL, Banfield JF (2004) Metabolically active eukaryotic communities in extremely acidic mine drainage. Appl Environ Microbiol 70:6264–6271

    Article  CAS  PubMed  Google Scholar 

  • Crous PW, Wingfield MJ (1997) New species of Mycosphaerella occurring on Eucalyptus leaves in Indonesia and Africa. Can J Bot 75:781–790

    Article  Google Scholar 

  • Crous PW, Groenewald JZ, Mansilla JP, Hunter GC, Wingfield MJ (2004) Phylogenetic reassessment of Mycosphaerella spp and their anamorphs occurring on Eucalyptus. Stud Mycol 50:195–214

    Google Scholar 

  • Crous PW, Wingfield MJ, Mansilla JP, Alfenas AC, Groenewald JZ (2006) Phylogenetic reassessment of Mycosphaerella spp and their anamorphs occurring on Eucalyptus. Stud Mycol 55:99–131

    Article  PubMed  Google Scholar 

  • Crous PW, Braun U, Groenewald JZ (2007a) Mycosphaerella is polyphyletic. Stud Mycol 58:1–32

    Article  CAS  PubMed  Google Scholar 

  • Crous PW, Summerell BA, Carnegie A, Mohammed C, Himaman W, Groenewald JZ (2007b) Foliicolous Mycosphaerella spp and their anamorphs on Corymbia and Eucalyptus. Fungal Divers 26:143–185

    Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Hölker U, Bend J, Pracht R, Müller T, Tetsch L, de Hoog GS (2004) Hortaea acidophila, a new acidophilic black yeast from lignite. Antonie Van Leeuwenhoek 86:287–294

    Article  PubMed  Google Scholar 

  • Ivarson KC, Morita H (1982) Single-cell protein production by the acid-tolerant fungus Scytalidium acidophilum from acid hydrolysates of waste paper. Appl Environ Microbiol 43:643–647

    CAS  PubMed  Google Scholar 

  • Johnson DB (1998) Biodiversity and ecology of acidophilic microorganisms. FEMS Microbiol Ecol 27:307–317

    Article  CAS  Google Scholar 

  • Kane J, Summerbell RC (1987) Sodium chloride as aid in identification of Phaeoannellomyces werneckii and other medically important dematiaceous fungi. J Clin Microbiol 25:944–946

    CAS  PubMed  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  CAS  PubMed  Google Scholar 

  • Luo H, Wang Y, Wang H, Yang J, Yang Y, Huang H, Yang P, Bai Y, Shi P, Fan Y, Yao B (2008) A novel highly acidic β-mannanase from the acidophilic fungus Bispora sp. MEY-1: gene cloning and overexpression in Pichia pastoris. Appl Microbiol Biotechnol 82:453–461

    Article  PubMed  Google Scholar 

  • Müller E, Oehrens E (1982) On the genus Teratosphaeria (Ascomycetes). Sydowia 35:138–142

    Google Scholar 

  • Nakagiri A, Tubaki K (1982) A new marine ascomycete and its anamorph from Japan. Trans Mycol Soc Jpn 23:101–110

    Google Scholar 

  • O’Donnell K (1993) Fusarium and its near relatives. In: Reynolds DR, Taylor JW (eds) The fungal holomorph: mitotic meiotic and pleomorphic speciation in fungal systematics. CAB International, Wallingford, pp 225–233

    Google Scholar 

  • Perrière G, Gouy M (1996) WWW-Query: an on-line retrieval system for biological sequence banks. Biochimie 78:364–369

    Article  PubMed  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  • Schleper C, Pühler G, Kühlmorgen B, Zillig W (1995) Life at extremely low pH. Nature 375:741–742

    Article  CAS  PubMed  Google Scholar 

  • Selbmann L, de Hoog GS, Zucconi L, Isola D, Ruisi S, Gerrits van den Ende AHG, Ruibal C, De Leo F, Urzì C, Onofri S (2008) Drought meets acid: three new genera in a dothidealean clade of extremotolerant fungi. Stud Mycol 61:1–20

    Article  CAS  PubMed  Google Scholar 

  • Sigler L, Carmichael JW (1974) A new acidophilic Scytalidium. Can J Microbiol 20:267–268

    Article  CAS  PubMed  Google Scholar 

  • Sletten O, Skinner CE (1948) Fungi capable of growing in strongly acid media and in concentrated copper sulfate solutions. J Bacteriol 56:679–681

    CAS  Google Scholar 

  • Starkey RL, Waksman SA (1943) Fungi tolerant to extreme acidity and high concentrations of copper sulfate. J Bacteriol 45:509–519

    CAS  PubMed  Google Scholar 

  • Sterflinger K (1998) Temperature and NaCl-tolerance of rock-inhabiting meristematic fungi. Antonie Van Leeuwenhoek 74:271–281

    Article  CAS  PubMed  Google Scholar 

  • Taylor JE, Groenewald JZ, Crous PW (2003) A phylogenetic analysis of Mycosphaerellaceae leaf spot pathogens of Proteaceae. Mycol Res 107:653–658

    Article  CAS  PubMed  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  CAS  PubMed  Google Scholar 

  • Ueda K, Mikata K (1999) A group I intron in the nuclear 18S rRNA gene of the yeast-like fungus, Arxula terrestris. Inst Ferment Osaka Res Commun 19:15–21

    Google Scholar 

  • Vilgalys L, Hester M (1990) Rapid genetic identification and mapping enzymatically amplified ribosomal DNA from several Cryptococcus species. Am Soc Microbiol 172:4238–4246

    CAS  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Inns MA, Gelfaud DH, Sninsky JJ, White TJ (eds) PCR protocols a guide to methods and applications. Academic Press, San Diego, pp 315–322

    Google Scholar 

  • Zalar P, de Hoog GS, Gunde-Cimerman N (1999) Ecology of halotolerant dothideaceous black yeasts. Stud Mycol 43:38–48

    Google Scholar 

  • Zettler LA, Gomez F, Zettler E, Keenan B, Amils R, Sogin M (2002) Eukaryotic diversity in Spain’s river of fire. Nature 417:137

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba, 292-0818, Japan

    Atsushi Yamazaki, Kyoko Toyama & Akira Nakagiri

Authors
  1. Atsushi Yamazaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kyoko Toyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Akira Nakagiri
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Atsushi Yamazaki.

About this article

Cite this article

Yamazaki, A., Toyama, K. & Nakagiri, A. A new acidophilic fungus Teratosphaeria acidotherma (Capnodiales, Ascomycota) from a hot spring. Mycoscience 51, 443–455 (2010). https://doi.org/10.1007/s10267-010-0059-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10267-010-0059-2

Keywords

Search

Navigation