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A new species, Aphanomyces salsuginosus sp. nov., isolated from ice fish Salangichthys microdon

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Mycoscience

Abstract

A species of Aphanomyces was isolated from the ice fish Salangichithys microdon living in brackish water in Japan. White cotton-like growth was found on the heads and fins of the fish. Hyphae penetrated into the dermal layers, subcutaneous tissues, muscular layers, and cartilaginous tissue of the mandible and maxilla; these hyphae were associated with cellular debris and lesions in host tissue. White fluffy colonies from subcultures of these growths were isolated on glucose–yeast agar plates with 0.5% sodium chloride (NaCl). These isolates consisted of delicate, slightly wavy, and moderately branched hyphae. Zoosporangia were isodiametric with the vegetative hyphae. Oogonia were abundant and approximately 21–33 μm in diameter, with irregular short papillae. Generally they were spherical or subspherical and only rarely pyriform. Individual oogonia usually contained a single oospore, which was spherical and 19–27 μm in diameter, with a large shiny vesicle. Antheridial branches, when present, were usually androgynous; however, they were sometimes monoclinous or diclinous. The optimal growth temperature of the isolates was 20°C, and cultures grew well at low salinity (0–0.5% NaCl). Phylogenic analysis based on the internal transcribed space 1-5.8S-ITS 2 of the ribosomal RNA gene indicates that these isolates will be an as-yet unidentified species of Aphanomyces.

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References

  • Abliz P, Fukushima K, Takizawa K, Nishimura K (2004) Identification of pathogenic dematiaceous fungi and related taxa based on large subunit ribosomal DNA D1/D2 domain sequence analysis. FEMS Immunol Med Microbiol 40:41–49

    Article  CAS  PubMed  Google Scholar 

  • Andrew TG, Huchzermeyer KD, Mbeha BC, Nengu SM (2008) Epizootic ulcerative syndrome affecting fish in the Zambezi river system in southern Africa. Vet Rec 163:629–631

    Article  CAS  PubMed  Google Scholar 

  • Baldoc FC, Blazer V, Callinan R, Hatai K, Karunasagar I, Mohan CV, Bondad-Reantaso MG (2005) Outcomes of a short expert consultation on epizootic ulcerative syndrome (EUS): reexamination of causal factors, case definition and nomenclature. In: Walker P, Bondad-Reantaso MG (eds) Diseases in Asian aquaculture V. Fish Health Section, Asian Fisheries Society, Manila, pp 555–585

    Google Scholar 

  • Ballesteros I, Martín MP, Diéguez-Uribeondo J (2006) First isolation of Aphanomyces frigidophilus (Saprolegniales) in Europe. Mycotaxon 95:335–340

    Google Scholar 

  • Dick MW (2001) The saprolegniales. In: Straminipilous fungi: systematics of the Peronosporomycetes including accounts of the marine Straminipilous Protists, the Plasmodiophorids and similar organisms. Kluwer Academic Publisher. Dordrecht, pp 160–165

  • Diéguez-Uribeondo J, García MA, Cerenius L, Kozubíková E, Ballesteros I, Windels C, Weiland J, Kator H, Söderhäll K, Martín MP (2009) Phylogenetic relationships among plant and animal parasites, and saprotrophs in Aphanomyces (Oomycetes). Fungal Genet Biol 46:365–376

    Article  PubMed  Google Scholar 

  • Dykstra MJ, Noga EJ, Levine JF, Moye DW (1986) Characterization of the Aphanomyces species involved with ulcerative mycosis (UM) in menhaden. Mycologia 78:664–672

    Article  Google Scholar 

  • Fraser GC, Millar SD, Calder LM (1992) Aphanomyces species associated with red spot disease: an ulcerative disease of estuarine fish from eastern Australia. J Fish Dis 15:173–181

    Article  Google Scholar 

  • Harada Y, Kuwamura K, Kinoshita I, Tanaka M, Tagawa M (2005) Histological observation of the pituitary-thyroid axis of a neotenic fish (the ice fish, Salangichtys microdon). Fish Sci 71:115–121

    Article  CAS  Google Scholar 

  • Hatai K (1980) Studies on pathogenic agents of saprolegniasis in fresh water fishes. Spec Rep Nagasaki Pref Inst Fish 8:1–95

    Google Scholar 

  • Hatai K (1989) Fungal pathogens/parasites of aquatic animals. In: Austin B, Austin DA (eds) Methods for the microbiological examination of fish and shellfish. Ellis Horwood Ltd., West Susssex, pp 240–272

    Google Scholar 

  • Hatai K, Nakamura K, Rha SA, Yuasa K, Wada S (1994) Aphanomyces infection in Dwarf Gourami (Colisa lalia). Fish Pathol 29:95–99

    Google Scholar 

  • Hendrickson DA (1985) Reagents and stains. In: Lennette EH (ed) Manual of clinical microbiology, 4th edn. American Society for Microbiology, Washington, pp 1093–1107

    Google Scholar 

  • Hoshina T, Sano T, Sunayama M (1960) Studies on the saprolegniasis of eel. J Tokyo Univ Fish 47:59–79

    Google Scholar 

  • Jeanmougin F, Thomson JD, Gouy M, Higgins DG, Gibson TJ (1998) Multiple sequence alignment with Clustal X. Trend Biochem Sci 23:403–405

    Article  CAS  PubMed  Google Scholar 

  • Johnson TW Jr, Seymour RL, Padgett DE (2002) Biology and the systematics of the Saprolegniaceae. http://dl.uncw.edu/digilib/biology/fungi/taxonomy%20and%20systematics/padgett%20book/

  • Johnson RA, Zabrecky J, Kiryu Y, Shields JD (2004) Infection experiments with Aphanomyces invadans in four species of estuarine fish. J Fish Dis 27:287–295

    Article  CAS  PubMed  Google Scholar 

  • Kitancharoen N, Hatai K (1997) Aphanomyces frigidophilus sp nov. from eggs of Japanese char, Salvelinus leucomaens. Mycoscience. Mycoscience 38:135–140

    Article  Google Scholar 

  • Kurtzman CP, Robnett CJ (1997) Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 50 end of the large-subunit (26S) ribosomal DNA gene. J Clin Microbiol 35:1216–1223

    CAS  PubMed  Google Scholar 

  • Levenfors JP, Fatehi J (2004) Molecular characterization of Aphanomyces species associated with legumes. Mycol Res 108:682–689

    Article  CAS  PubMed  Google Scholar 

  • Lilley JH, Callinan RB, Chinabut S, Kanchanakhan S, MacRae IH, Phillips MJ (1998) Epizootic ulcerative syndrome (EUS) technical handbook. Aquatic Animal Health Research Institute, Department of fisheries, Bangkok

    Google Scholar 

  • Miyazaki T, Egusa S (1973) Studies on mycotic granulomatosis in fresh water fishes-II Mycotic granulomatosis in ayu, Plecoglossus. Fish Pathol 7:25–133

    Google Scholar 

  • Noga EJ, Dykstra MJ (1986) Oomycete fungi associated with ulcerative mycosis in menhaden, Brevoortia tyrannus (Latrobe). J Fish Dis 9:47–53

    Article  Google Scholar 

  • Oidtmann B, Schaefers N, Cerenius L, Soderhall K, Hoffmann RW (2004) Detection of genomic DNA of the crayfish plague fungus Aphanomyces astaci (Oomycete) in clinical samples by PCR. Vet Microbiol 100:269–282

    Article  CAS  PubMed  Google Scholar 

  • Royo F, Andersson G, Bangyeekhun E, Muzquiz JL, Soderhall K, Cerenius L (2004) Physiological and genetic characterisation of some new Aphanomyces strains isolated from freshwater crayfish. Vet Microbiol 104:103–112

    Article  CAS  PubMed  Google Scholar 

  • Scott WW (1961) A monograph of the genus Aphanomyces. Virginia Agri Exp Stat Tech Bull 151

  • Shah KL, Jha BC, Jhingran AG (1977) Observations on some aquatic phycomycetes pathogenic to eggs and fry of freshwater fish and prawn. Aquaculture 12:141–147

    Article  Google Scholar 

  • Shanor L, Saslow HB (1944) Aphanomyces as a fish parasite. Mycologia 36:413–415

    Article  Google Scholar 

  • Sinmuk S, Suda H, Hatai K (1996) Aphanomyces infection in juvenile soft-shelled turtle, Pelodiscus sinensis, imported from Singapore. Mycoscience 37:249–254

    Article  Google Scholar 

  • Sparrow FK (1960) Aquatic Phycomycetes, 2nd edn. Univ. Michigan Press, Ann Arbor

    Google Scholar 

  • Srivastava GC, Srivastava RC (1976) A note on the destruction of the eggs of Cyprinus carpio var. Communis by the members of Saprolegniaceae. Sci Cult 42:612–614

    Google Scholar 

  • Swofford DL (2001) PAUP phylogenetic analysis using parsimony (and other methods) (version 4.0) Sinauer Associates, Sunderland

  • Unestam T (1965) Studies on the crayfish plague fungus Aphanomyces astaci Part I. Some factors affecting growth in vitro. Physiol Plant 18:483–505

    Article  Google Scholar 

  • Unestem T (1972) On the host range and origin of the crayfish plague fungus. Rep Inst Freshw Res Drottningholm 52:192–198

    Google Scholar 

  • Vralstad T, Knutsen AK, Tengs T, Holst-Jensen A (2009) A quantitative TaqManR MGB real-time polymerase chain reaction based assay for detection of the causative agent of crayfish plague Aphanomyces astaci. Vet Microbiol 137:145–155

    Article  Google Scholar 

  • Willoughby LG, Robert RJ (1994) Improved methodology for isolation of the Aphanomyces fungal pathogen of epizootic ulcerative syndrome (EUS) in Asian fish. J Fish Dis 17:237–248

    Article  Google Scholar 

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Acknowledgments

We sincerely thank the technical support of Ms. Mihoko Kobayashi and Ms. Shinako Suzuki from our department and Ms. Kyoko Yarita from Chiba University, and the staff members of the aquarium, Gobius, Shinjiko Nature Museum, Shimane, Japan. This study was partially supported by the Special Research Fund for Emerging and Re-emerging Infections of the Ministry of Health, Welfare and Labor (Grant No. H-18-Shinkou-8. and No. H-21-Shinkou-4) for Dr. Ayako Sano.

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Authors and Affiliations

  1. Laboratory of Fish Diseases, Nippon Veterinary and Life Science University, 1-7-1, Kyonan-Cho, Musashino, Tokyo, 180-8602, Japan

    Daisuke Takuma, Shinpei Wada, Osamu Kurata & Kishio Hatai

  2. Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba, Japan

    Ayako Sano

Authors
  1. Daisuke Takuma
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  2. Ayako Sano
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  3. Shinpei Wada
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  4. Osamu Kurata
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  5. Kishio Hatai
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Correspondence to Kishio Hatai.

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Takuma, D., Sano, A., Wada, S. et al. A new species, Aphanomyces salsuginosus sp. nov., isolated from ice fish Salangichthys microdon . Mycoscience 51, 432–442 (2010). https://doi.org/10.1007/s10267-010-0058-3

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  • DOI: https://doi.org/10.1007/s10267-010-0058-3

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