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Discrimination of Tricholoma species by species-specific ITS primers

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Mycoscience

Abstract

The ITS region of ectomycorrhizal fungi was analyzed, and species-specific PCR primers were designed for 8 ectomycorrhizal Tricholoma species. Although a high degree of intraspecific homology was observed, interspecific variation was sufficient to design species-specific primers based on sequence of the ITS region. PCR amplification with the specific primers generated fragments of the expected sizes from DNA extracted from the strains of each species but gave no amplified products from the strains of the other 16 species in eight genera. These results suggest that sequence of the ITS region is appropriate to be used for species-level identification of ectomycorrhizal fungi.

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Authors and Affiliations

  1. Laboratory of Forest Botany, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Kensuke Kikuchi & Norihisa Matsushita

  2. Laboratory of Forest Microbiology, College of Bioresource Sciences, Nihon University, Kanagawa, Japan

    Kazuo Suzuki

Authors
  1. Kensuke Kikuchi
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  2. Norihisa Matsushita
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  3. Kazuo Suzuki
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Correspondence to Kensuke Kikuchi.

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Kikuchi, K., Matsushita, N. & Suzuki, K. Discrimination of Tricholoma species by species-specific ITS primers. Mycoscience 48, 316–320 (2007). https://doi.org/10.1007/s10267-007-0368-2

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  • DOI: https://doi.org/10.1007/s10267-007-0368-2

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