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Transformation of Lyophyllum decastes by particle bombardment

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Mycoscience

Abstract

The basidiomycete Lyophyllum decastes was transformed by means of particle bombardment. We isolated five transformants under twelve conditions differing in the two parameters of target distance and helium pressure. The transformation frequency was one transformant/µg DNA. In the transformants, plasmid DNAs were integrated into the genomic DNA and stably maintained. This is the first report on transformation of L. decastes by particle bombardment.

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Authors and Affiliations

  1. Department of Applied Microbiology, Forestry and Forest Products Research Institute, Ibaraki, 305-8687, Japan

    Masahide Sunagawa, Hitoshi Murata, Yasumasa Miyazaki & Masaya Nakamura

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  1. Masahide Sunagawa
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  2. Hitoshi Murata
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  3. Yasumasa Miyazaki
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  4. Masaya Nakamura
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Correspondence to Masahide Sunagawa.

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Sunagawa, M., Murata, H., Miyazaki, Y. et al. Transformation of Lyophyllum decastes by particle bombardment. Mycoscience 48, 195–197 (2007). https://doi.org/10.1007/s10267-007-0345-9

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  • DOI: https://doi.org/10.1007/s10267-007-0345-9

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