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Effect of initial periodontal therapy on metallothionein levels in smokers and non-smokers with periodontitis

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Abstract

The aim of this study was to determine the effect of non-surgical periodontal therapy (NSPT) on mRNA expression of metallothionein (MT) and its levels in serum, saliva and gingival crevicular fluid (GCF) of smokers (S) and non-smokers (NS) with periodontitis (P).

A total of 100 participants were included: 48 periodontally healthy (PH) subjects (24 S [PH + S] and 24 NS [PH + NS]) and 52 patients with P (27 S [P + S] and 25 NS [P + NS]). Clinical parameters were recorded, and biofluids (serum, saliva and GCF) and gingival tissue samples were obtained at baseline in all groups and 3 months after NSPT in P groups. MT levels in biofluids were determined by ELISA. In gingival tissues, MT-mRNA expression was quantified using real-time PCR. mRNA expression of MT and its levels in biofluids were significantly higher in P + S compared to other groups, and the differences between P + NS and PH + S were non-significant. A significant decrease was observed for MT levels in biofluids, and MT-mRNA expression in periodontitis patients after NSPT. In conclusion, smoking and periodontitis are associated with higher MT expression which decreases after NSPT. MT as an oxidative stress biomarker and its therapeutic role in periodontitis should be investigated in future studies.

Clinical trial registration: The study was prospectively registered at Clinical Trials Registry—India (ctri.nic.in) as CTRI/2018/08/015427 on August 23, 2018.

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Data availability

The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

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Funding

Sources of financial support: The research was funded by Institutional grant from AIIMS, New Delhi, India.

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Authors and Affiliations

  1. Division of Periodontics, Centre for Dental Education and Research, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110029, India

    Vikender Singh Yadav & Kanika Makker

  2. PRIMSR Hospital, SRM University, Sonipat, Haryana, India

    Anu Bhatia

  3. Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India

    Rakhee Yadav, Dhiraj Kumar Singh & Riyaz Ahmad Mir

Authors
  1. Vikender Singh Yadav
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  2. Anu Bhatia
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  3. Rakhee Yadav
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  4. Kanika Makker
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  5. Dhiraj Kumar Singh
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  6. Riyaz Ahmad Mir
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Contributions

Conceptualization: VSY, AB; Formal Analysis: AB, RY, KM; Investigation: RY, DKS; Methodology: VSY, KM; Project Administration: VSY, RAM; Writing—Original Draft: VSY, AB, KM; Writing—Review and Editing: RY, DKS, RAM.

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Correspondence to Vikender Singh Yadav.

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Ethical approval (IEC-271/04.07.2017) was granted by the Institutional Ethics committee on human subjects.

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A written informed consent was obtained from all the participants after explaining the purpose and procedure of the study. 

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Yadav, V.S., Bhatia, A., Yadav, R. et al. Effect of initial periodontal therapy on metallothionein levels in smokers and non-smokers with periodontitis. Odontology (2024). https://doi.org/10.1007/s10266-024-00937-x

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