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Ultrasonic activation of the endodontic sealer enhances its intratubular penetration and bond strength to irradiated root dentin

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Abstract

To evaluate the effect of ultrasonic activation of the endodontic sealer on its intratubular penetration and bond strength to irradiated root dentin. Forty human teeth were distributed into 4 groups (n = 10), according to the radiation therapy (RT) exposure—70 Gy—and ultrasonic activation (UA) of the endodontic sealer: RT/UA—irradiated teeth and sealer UA; RT/no-UA—irradiated teeth and no sealer UA; no-RT/UA—non-irradiated teeth and sealer UA and no-RT/no-UA—non-irradiated teeth and no sealer UA. Push-out bond strength test was performed in a Universal Testing Machine. Failure modes and adhesive interface were analyzed under Scanning Electron Microscopy. The data were statistically compared (two-way-ANOVA and posthoc Games-Howell test; Fisher’s exact test − α = 5%). The different experimental conditions (radiation and UA) and the root third had a significant effect on push-out bond strength, and the interaction of these factors was significant (p < 0.05). UA of the sealer significantly increased its bond strength to both irradiated and non-irradiated dentin (p < 0.05). The irradiated groups mostly presented adhesive-type failure of the sealer (p < 0.01). Regardless of the irradiation, the ultrasonically activated groups showed a more homogeneous adhesive interface, with the presence of sealer tags in greater density and depth. Ultrasonic activation enhanced the intratubular penetration and the bond strength of the endodontic sealer to irradiated dentin. The impact of ultrasonic activation of the endodontic sealer on teeth undergoing radiotherapy is a gap in the scientific literature that needs to be bridged.

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Acknowledgements

The authors would like to thank the Department of Radiotherapy of the Oncology Research Centre (CEPON-Florianópolis, SC, Brazil) for the irradiation of the teeth.

Funding

The present study was funded by the National Council for Scientific and Technological Development (Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq).

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Authors and Affiliations

  1. Department of Dentistry, Endodontics Division, Health Sciences Centre, Federal University of Santa Catarina, Campus Reitor João David Ferreira Lima, Florianópolis, Santa Catarina, 88040-900, Brazil

    Luana Duart Jordani, Amanda Freitas da Rosa, Luiz Carlos de Lima Dias-Junior, Julia Menezes Savaris, Cleonice da Silveira Teixeira & Lucas da Fonseca Roberti Garcia

  2. Department of Radiotherapy, Oncology Research Centre (CEPON), Florianópolis, Santa Catarina, Brazil

    Mariana Comparotto Minamisako, Luciano Roberto da Silva & Marcio Toshio Umeda Takashima

  3. Department of Diagnosis and Oral Health, Endodontics Division, University of Louisville, Louisville, KY, USA

    Eduardo Antunes Bortoluzzi

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Contributions

LDJ: Conceptualization; Data curation; Investigation; Methodology; Writing—original draft; Writing—review and editing; AFdaR: Validation; Visualization; LCLD-J: Formal analysis; JMS: Roles/Writing—original draft; Writing—review and editing; MCM: Resources; LRdaS: Methodology; Resources; MTUT: Methodology; Resources; EAB: Validation; Visualization; CdaST: Roles/Writing-original draft; Writing-review and editing; LdaFRG: Conceptualization; Supervision; Project administration; Visualization; Writing-review and editing. The authors of the present study declare that they have contributed significantly and are in agreement with the manuscript content.

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Correspondence to Lucas da Fonseca Roberti Garcia.

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The present study was conducted after the proper approval from the (CAAE: 46526221.6.1001.0121).

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Jordani, L.D., da Rosa, A.F., Dias-Junior, L.C.d. et al. Ultrasonic activation of the endodontic sealer enhances its intratubular penetration and bond strength to irradiated root dentin. Odontology 112, 917–928 (2024). https://doi.org/10.1007/s10266-023-00888-9

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