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Analysis of the subcellular location of lncRNA SLC16A1-AS1 and its interaction with premature miR-5088-5p in oral squamous cell carcinoma

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Abstract

SLC16A1-AS1 is a newly identified lncRNA with different roles in different cancers. MiR-5088-5p is an oncogenic miRNA in breast cancer. However, their participation in oral squamous cell carcinoma (OSCC) is unknown. We predicted the interaction between SLC16A1-AS1 and miR-5088-5p, and this study was carried out to explore the crosstalk between them in OSCC. A total of 56 OSCC patients donated OSCC and paired non-tumor tissues, which were used to detect the differential expression of SLC16A1-AS1 and miR-5088-5p (mature and premature). Analysis of the subcellular location of SLC16A1-AS1 in OSCC cells and its direct interaction with premature miR-5088-5p was performed with cellular fractionation assay and RNA pull-down assay, respectively. The involvement of SLC16A1-AS1 in miR-5088-5p maturation was studied with overexpression assay. BrdU assay was performed to detect cell proliferation after transfection. OSCC tissue samples exhibited decreased expression levels of SLC16A1-AS1 and premature miR-5088-5p, but increased the expression levels of mature miR-5088-5p. SLC16A1-AS1 was detected in both nucleus and cytoplasm samples of OSCC cells and its direct interaction with premature miR-5088-5p was confirmed. Overexpression of SLC16A1-AS1 in OSCC cells resulted in inhibited maturation of miR-5088-5p. SLC16A1-AS1 suppressed the enhancing effects of miR-5088-5p on cell proliferation. SLC16A1-AS1 was downregulated in OSCC and it may inhibit cell proliferation by suppressing maturation of miR-5088-5p.

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Data availability

The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to their containing information that could compromise the privacy of research participants.

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Author notes

  1. Tiecheng Li and Di Wang contributed equally to the work.

Authors and Affiliations

  1. Department of Stomatology, Daqing Oilfiled General Hospital, Daqing, 163000, Heilongjiang, People’s Republic of China

    Tiecheng Li

  2. Department of Prosthodontics, Daqing Oilfiled General Hospital, Daqing, 163000, Heilongjiang, People’s Republic of China

    Di Wang

  3. Department of Geriatrics, Daqing Oilfiled General Hospital, No. 9, Sartu District, Daqing, 163000, Heilongjiang, People’s Republic of China

    Shuo Yang

Authors
  1. Tiecheng Li
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  2. Di Wang
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Contributions

SY: study concepts, literature research, clinical studies, data analysis, experimental studies, manuscript writing and review; TL, DW: study design, literature research, experimental studies and manuscript editing; TL: definition of intellectual content, clinical studies, data acquisition and statistical analysis; DW: data acquisition, manuscript preparation and data analysis. All authors have read and approve the submission of the manuscript.

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Correspondence to Shuo Yang.

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All other authors have no conflicts of interest. We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work submitted.

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All patients signed the written informed consent. All producers were approved by Daqing Oilfiled General Hospital Ethics Committee. Procedures operated in this research were completed in keeping with the standards set out in the Announcement of Helsinki and laboratory guidelines of research in China.

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10266_2022_712_MOESM1_ESM.tif

Supplemental Figure 1 In situ hybridization analysis of the expression of SLC16A1-AS1 and premature miR-5088-5p in paired OSCC samples (TIF 23430 KB)

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Li, T., Wang, D. & Yang, S. Analysis of the subcellular location of lncRNA SLC16A1-AS1 and its interaction with premature miR-5088-5p in oral squamous cell carcinoma. Odontology 111, 41–48 (2023). https://doi.org/10.1007/s10266-022-00712-w

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  • DOI: https://doi.org/10.1007/s10266-022-00712-w

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