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WDR5 promotes the tumorigenesis of oral squamous cell carcinoma via CARM1/β-catenin axis

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Abstract

Oral squamous cell carcinoma (OSCC) is a malignancy all over the world. WD repeat domain 5 (WDR5) is involved in cancer progression. In addition, it was reported that WDR5 is upregulated in head and neck cancer, while its role in OSCC is unknown. First, the expression of WDR5 in oral cancer tissues and cells was examined by qRT-PCR, IHF and western blot. CCK-8 assay was performed to test the cell viability. Cell migration was assessed by transwell assay. Knocking down WDR5 or CARM1 in oral cancer cells to detect its function on cancer growth, WDR5 and CARM1 were significantly upregulated in OSCC. Silencing WDR5 suppressed OSCC cell viability and migration. CARM1 level in OSCC cells was significantly inhibited by WDR5 downregulation, and CARM1 elevation could rescue the effect of WDR5 knockdown on tumorigenesis of OSCC. Moreover, silencing of WDR5 notably inactivated β-catenin signaling pathway, while this phenomenon was restored by CARM1 overexpression. Silencing of WDR5 attenuated the tumorigenesis of OSCC via CARM1/β-catenin axis. Thus, WDR5 might be a target for OSCC treatment.

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Abbreviations

WDR5:

WD repeat domain 5

CARM1:

Co-activator-associated arginine methyltransferase 1

OSCC:

Oral squamous cell carcinoma

PRMT:

Protein arginine methyltransferase

HOK:

Human normal oral keratinocytes

ZEB1:

Zinc finger E-box-binding homeobox 1

H3K4me3:

Histone H3 lysine 4 tri-methylation

LSD1:

Lysine-specific demethylase 1

R838:

Methylation site

USP7:

Ubiquitin-specific protease 7

PRDX1:

Peroxiredoxin 1

FBS:

Fatal bovine serum

IHC:

Immunocytochemistry

PBS:

Phosphate-buffered saline

DAB:

Diaminobenzidine

RT-qPCR:

Real-time reverse transcriptase quantitative PCR

PVDF:

Polyvinylidene difluoride

SD:

Standard deviation

ANOVA:

One-way analysis of variance

PARP1:

Polymerase 1

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (No. 81800989).

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Authors and Affiliations

  1. State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, No. 14 Renmin South Road, Chengdu, 610065, People’s Republic of China

    Xiao-Ying Lyu, Yu-Sen Shui, Liang Wang, Qing-Song Jiang, Ling-Xi Meng & Ran Yang

  2. Sichuan University, Chengdu, 610065, People’s Republic of China

    Hao-Yuan Zhan

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  1. Xiao-Ying Lyu
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Correspondence to Ran Yang.

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Lyu, XY., Shui, YS., Wang, L. et al. WDR5 promotes the tumorigenesis of oral squamous cell carcinoma via CARM1/β-catenin axis. Odontology 110, 138–147 (2022). https://doi.org/10.1007/s10266-021-00649-6

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  • DOI: https://doi.org/10.1007/s10266-021-00649-6

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