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Universal adhesive: the effect of different simulated pulpal pressure fluids and bonding modes to dentin

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Abstract

The aim of this study was to evaluate the effect of SPP with either fetal bovine serum (FBS) or deionized water (DW) on the bond strength (μTBS) of a Universal adhesive to dentin, in both etch-and-rinse (ER) and self-etch (SE) modes. The kinematic viscosity (cSt) of FBS and DW was measured at 25 °C ± 0.1 ºC. Seventy-two sound human molars were sectioned and randomly divided into three groups according to the SPP conditions: (1) Control (0 cm H2O), (2) SPP (15 cm H2O) with FBS, (3) SPP (15 cm H2O) with DW. Each group was subdivided (n = 10) based on the bonding modes: ER (37% phosphoric acid + ScothBond Universal Adhesive) or SE (ScothBond Universal Adhesive). Samples were then submitted to μTBS. Data were analyzed by Student’s t test, two-way ANOVA and Tukey tests (p < 0.05). The cSt results showed that DW (23.59 ± 0.39) had significantly higher values than FBS (22.33 ± 0.06). With regard to SPP, the control group (36.1 MPa) had significantly higher values of μTBS when compared to the SPP using FBS (31.06 MPa) and SPP with DW (26.55 MPa). According to ANOVA, the bonding modes and the interaction of simulated pulpal pressure (SPP) did not statistically influence the results (p < 0.05). The presence of SPP reduced the bond strength of Universal adhesive to dentin. DW during SPP had significantly reduced bonding values when compared to FBS. Bonding strategies were not affected by SPP when evaluated in a short period of time (24 h).

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Funding

The work was supported by the Department Restorative Dentistry, Institute of Science and technology. UNESP–Univ Estadual Paulista, São José dos Campos, SP, Brazil.

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Authors and Affiliations

  1. Anhanguera Faculty of São José dos Campos, São José dos Campos, SP, Brazil

    Lucélia Lemes Gonçalves & Tânia Mara Da Silva

  2. Department of Clinical Sciences Restorative, Faculty of Dentistry, University of Toronto, Toronto, ON, Canada

    Anuradha Prakki

  3. Department of Prosthodontics, Federal University of Espírito Santo, Vitória, ES, Brazil

    Daphne Camara Barcellos

  4. Department Restorative Dentistry, Institute of Science and Technology, UNESP–Univ Estadual Paulista, São José dos Campos, SP, Brazil

    Taciana Marco Ferraz Caneppele & Sérgio Eduardo de Paiva Gonçalves

  5. Center for Natural and Human Sciences, Federal University of ABC, Santo André, SP, Brazil

    Hueder Paulo Moisés De Oliveira

  6. Departamento de Odontologia Restauradora, Instituto de Ciência e Tecnologia, UNESP–Univ Estadual Paulista, Campus de São José dos Campos, Av. Engenheiro Francisco José Longo, 777, Jardim São Dimas, São José dos Campos, SP, 12245-000, Brazil

    Sérgio Eduardo de Paiva Gonçalves

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  1. Lucélia Lemes Gonçalves
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Gonçalves, L.L., Da Silva, T.M., Prakki, A. et al. Universal adhesive: the effect of different simulated pulpal pressure fluids and bonding modes to dentin. Odontology 110, 62–69 (2022). https://doi.org/10.1007/s10266-021-00633-0

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