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Combined in silico analysis identified a putative tooth root formation-related gene, Chd3, which regulates DNA synthesis in HERS01a cells

Odontology volume 108, pages 386–395 (2020)Cite this article

Abstract

There exists a close connection between changes occurring in the teeth and those occurring in the jaw during the evolutionary process. In mammals, the roots of teeth are supported, along with periodontal ligaments and alveolar bones by a unique structure termed the gomphosis. In the present study, we performed combined in silico analysis using the information obtained from various DNA microarrays and identified 19 putative tooth root formation-related genes. Furthermore, quantitative PCR was performed on the candidate genes, Chd3 was confirmed as having sufficient expression levels in the early stage of tooth root formation and increased gene expression toward the middle stage. A high degree of Chd3 gene expression was observed in secretory ameloblasts and Hertwig’s epithelial root sheath (HERS), but low expression was observed in developing odontoblasts and stellate reticulum. The CHD3 foci were observed in the nucleus of the HERS01a cells. In addition, knockdown experiments using SiChd3 suggested the involvement of Chd3 in the suppression of DNA synthesis. These results suggested that Chd3 plays a role in DNA synthesis in HERS cells for promoting tooth root development.

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Abbreviations

EGF:

Epidermal growth factor

HERS:

Hertwig's epithelial root sheath

NuRD:

Nucleosome remodeling and deacetylase

Pgk1:

Phosphoglyceate kinase 1

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Acknowledgements

This work was supported by a Grant-in-Aid for Exploratory Research (Grant 25670831) from the Japan Society for the Promotion of Science.

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Affiliations

  1. Section of Molecular Craniofacial Embryology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan

    Yuki Date, Sachiko Iseki & Masato S. Ota

  2. Section of Oral Implantology and Regenerative Dental Medicine, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan

    Yuki Date, Hisatomo Kondo & Shohei Kasugai

  3. Department of Prosthodontics and Oral Implantology, School of Dentistry, Iwate Medical University, Morioka, Japan

    Hisatomo Kondo

  4. Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women’s University, 2-8-1, Mejirodai, Bunkyo-Ku, Tokyo, 112-8681, Japan

    Atsuko Yamashita & Masato S. Ota

Authors
  1. Yuki Date
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  2. Hisatomo Kondo
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  3. Atsuko Yamashita
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  4. Sachiko Iseki
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  5. Shohei Kasugai
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  6. Masato S. Ota
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Correspondence to Masato S. Ota.

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Date, Y., Kondo, H., Yamashita, A. et al. Combined in silico analysis identified a putative tooth root formation-related gene, Chd3, which regulates DNA synthesis in HERS01a cells. Odontology 108, 386–395 (2020). https://doi.org/10.1007/s10266-020-00489-w

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  • DOI: https://doi.org/10.1007/s10266-020-00489-w

Keywords

  • Tooth root
  • Transcriptome
  • Gene expression
  • siRNA
  • DNA synthesis