Histological healing after nonsurgical periodontal treatment with enamel matrix derivatives in canine experimental periodontitis
- 205 Downloads
The histological outcomes after nonsurgical periodontal treatment with enamel matrix derivatives (EMD) remain controversial. The present study evaluated periodontal wound healing after scaling and root planing (SRP) with subgingival application of EMD for treatment of experimental periodontitis. Periodontal breakdown was induced by applying silk ligatures around mandibular third and fourth premolars of six beagle dogs until radiographic bone loss progressed to approximately half of the root length. Probing pocket depth (PPD) and clinical attachment level (CAL) were proximally measured 2 weeks after ligature removal (baseline). Mesial and distal surfaces of the experimental teeth were subjected to SRP and randomized using a split-mouth design to subgingival application of EMD (test) or normal saline (control). PPD and CAL were re-evaluated at 11 weeks. Animals were sacrificed at 12 weeks for histological analyses. No significant differences were observed in PPD and CAL between both groups at baseline and at 11 weeks. Histologically, test sites exhibited a greater amount of new cementum than that did the control sites (p < 0.01). Moreover, the control sites revealed increased epithelial downgrowth compared with the test sites: (p < 0.05). On the other hand, no intergroup differences were detected in terms of bone position, connective tissue attachment, gingival recession, and planed root length. This study suggested that EMD has an increased potential to support formation of new cementum with decreased epithelial downgrowth when used as an adjunct to nonsurgical periodontal treatment.
KeywordsRoot planing Enamel matrix derivatives Nonsurgical periodontal treatment Histology Animal studies
This study was supported by the Japan Society for the Promotion of Science (JSPS KAKENHI Grant Numbers JP16751919 and 17K11980). We would like to thank Professor Atsuhiro Kinoshita (Department of Educational Media Development, Institute for Library and Media Information Technology, Tokyo Medical and Dental University, Tokyo, Japan) for his kind help and scientific comments. The authors would like to thank Dr. Walter H. Meinzer II (Department of Periodontology, Tokyo Medical and Dental University) for the English language review.
This study was funded by the Japan Society for the Promotion of Science (JSPS KAKENHI Grant Numbers JP16751919 and 17K11980).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 7.Dragoo MR, Grant DA, Gutverg D, Stambaugh R. Experimental periodontal treatment in humans. I. Subgingival root planning with and without chlorhexidine gluconate rinses. Int J Periodontics Restor Dent. 1984;4:8–29.Google Scholar
- 9.Jepsen K, Jepsen S. Antibiotics/antimicrobials: systemic and local administration in the therapy of mild to moderately advanced periodontitis. Periodontol. 2000;2016(71):82–112.Google Scholar
- 10.Susin C, Fiorini T, Lee J, De Stefano JA, Dickinson DP, Wikesjo UM. Wound healing following surgical and regenerative periodontal therapy. Periodontol. 2000;2015(68):83–98.Google Scholar
- 11.Cortellini P, Tonetti MS. Clinical concepts for regenerative therapy in intrabony defects. Periodontol. 2000;2015(68):282–307.Google Scholar
- 16.Aspriello SD, Zizzi A, Spazzafumo L, Rubini C, Lorenzi T, Marzioni D, et al. Effects of enamel matrix derivative on vascular endothelial growth factor expression and microvessel density in gingival tissues of periodontal pocket: a comparative study. J Periodontol. 2011;82:606–12.CrossRefPubMedGoogle Scholar
- 32.Fujita T, Yamamoto S, Ota M, Shibukawa Y, Yamada S. Coverage of gingival recession defects using guided tissue regeneration with and without adjunctive enamel matrix derivative in a dog model. Int J Periodontics Restor Dent. 2011;31:247–53.Google Scholar