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Evaluation of chemerin and its receptors, ChemR23 and CCRL2, in gingival tissues with healthy and periodontitis

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Abstract

Chemerin is a chemoattractant protein that directs inflammatory cells that express its receptor chemokine receptor-like 1 (ChemR23) towards sites of inflammation. C–C chemokine receptor-like 2 (CCRL2), is the other receptor of chemerin, improves the interaction between chemerin and ChemR23. The aim of this study was to evaluate the expression of chemerin and its receptors in gingival tissues with healthy and periodontitis. Tissue biopsy samples were obtained from 20 patients with chronic periodontitis and from the gingiva of 20 healthy individuals undergoing a crown lengthening process. Quantitative real-time PCR (qPCR) was used to examine the mRNA expression of chemerin, ChemR23 and CCRL2. Additionally, protein expression was measured by immunohistochemistry. Both qPCR and immunohistochemistry results revealed that the expression of chemerin and ChemR23 was significantly higher in tissues with periodontitis than in healthy tissues (P = 0.001 and, P = 0.015, respectively). There were no significant differences between healthy tissues and those with periodontitis in terms of mRNA expression of CCRL2, whereas a more intense staining was observed in tissues with periodontitis. The mRNA expression levels of chemerin showed a positive correlation with plaque index, gingival index, probing pocket depth and clinical attachment level (r = 0.448, r = 0.460, r = 0.439 and, r = 0.459, respectively, P < 0.01). To the best of our knowledge, this study is the first to examine the expression of chemerin, ChemR23 and CCRL2 in gingival tissues. Our study suggests that chemerin may play a role in the pathogenesis of periodontitis by causing chemoattraction of immune cells that direct ChemR23 receptors to the site of inflammation.

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Authors and Affiliations

  1. Department of Periodontology, Center of Oral Health, Gülhane Medical Academy, Ankara, 06018, Turkey

    Erkan Özcan & N. Işıl Saygun

  2. Department of Immunology, Gülhane Medical Academy, Ankara, Turkey

    Rahşan Ilıkçı, Uğur Muşabak & Sait Yeşillik

  3. Department of Pathology, Gülhane Medical Academy, Ankara, Turkey

    Yıldırım Karslıoğlu

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  1. Erkan Özcan
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  2. N. Işıl Saygun
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  3. Rahşan Ilıkçı
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  4. Yıldırım Karslıoğlu
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  5. Uğur Muşabak
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  6. Sait Yeşillik
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Correspondence to Erkan Özcan.

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Funding

This study was funded by Gülhane Medical Academy Research Board, with project number AR-2014/36.

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The authors declare that they have no conflict of interest.

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Özcan, E., Saygun, N.I., Ilıkçı, R. et al. Evaluation of chemerin and its receptors, ChemR23 and CCRL2, in gingival tissues with healthy and periodontitis. Odontology 106, 29–36 (2018). https://doi.org/10.1007/s10266-017-0297-2

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  • DOI: https://doi.org/10.1007/s10266-017-0297-2

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