Odontology

, Volume 104, Issue 2, pp 123–135 | Cite as

Isolation, characterization and investigation of differentiation potential of human periodontal ligament cells and dental follicle progenitor cells and their response to BMP-7 in vitro

  • Yahya Açil
  • Fan Yang
  • Aydin Gulses
  • Mustafa Ayna
  • Jörg Wiltfang
  • Matthias Gierloff
Original Article

Abstract

The aim of this study was to assess the factors, mechanisms and the differences between periodontal ligament (PDL) cells and denta l follicle (DF) progenitor cells towards the osteoblastic/cementoblastic differentiation and to investigate the effects of BMP-7 on developmental (DF) and mature tissue-derived (PDL) cells, respectively. Primary cell culture of PDL cells and DF progenitor cells was performed. Osteogenic differentiation was evaluated using von Kossa, Alizarin Red S and immuno-histo-chemistry staining of osteocalcin. Gene expression pattern was evaluated via real-time PCR. A series of CD surface marks were tested using flow cytometry and fluorescence-activated cell-sorting analysis was performed. Real-time RT-PCR demonstrated similar gene expression pattern of PDL cells and DF progenitor cells: the expression of OPN and OCN significantly was elevated when incubated with osteogenic components, Runx2 was unaffected, and Osteorix was hardly expressed whether in basic medium or induction medium. In addition, BMP-7 induced osteoblast/cementoblast differentiation of PDLSCs and DF progenitor cells in a dose- and time-dependent manner, as reflected by enhanced Runx2 and (OCN) mRNA transcript expression. BMP-7 triggers PDL cells and DF progenitor cells to differentiate towards an osteoblast/cementoblast phenotype.

Keywords

BMP-7 Dental follicle Periodontal ligament Progenitor Differentiation 

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Copyright information

© The Society of The Nippon Dental University 2015

Authors and Affiliations

  • Yahya Açil
    • 1
  • Fan Yang
    • 2
  • Aydin Gulses
    • 3
  • Mustafa Ayna
    • 4
  • Jörg Wiltfang
    • 1
  • Matthias Gierloff
    • 1
  1. 1.Department of Oral and Maxillofacial SurgeryChristian Albrechts UniversityKielGermany
  2. 2.Affiliated Hospital of Stomatology, Medical CollegeZhejiang UniversityHangzhouChina
  3. 3.Centre for Oral Health and Dental ManagementMevki Military HospitalDiskapi AltindagTurkey
  4. 4.Private practiceDuisburgGermany

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