Abstract
This study aims to investigate the effect of alpha-mangostin on interleukin (IL)-6 and IL-8 expression in human gingival fibroblasts (HGFs). HGFs were challenged with Porphyromonas gingivalis LPS and then treated with various concentrations of alpha-mangostin. The cytotoxicity was determined using MTS assay and cytokine expressions were evaluated by Real-time PCR and ELISA. The results showed that 5 μg/ml P. gingivalis LPS and alpha-mangostin at 1 µg/ml or less did not affect the viability of HGFs. Alpha-mangostin reduced IL-6 and IL-8 mRNA and protein in P. gingivalis LPS-stimulated HGFs. These findings suggested that alpha-mangostin might be used as an adjunct to the periodontal therapy.
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Acknowledgments
The authors wish to thank Associate Professor Suchada Chutimaworapan, Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Thailand, for her support. The authors thank Professor Dr. Prasit Pavasant, Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, for his useful suggestions on the manuscript. We also thank Mr. Arthur Ian Simpson, a former instructor at the Naresuan University International College, Naresuan University, for kindly editing the English. This work was supported by the Faculty of Dentistry, Naresuan University (Research grants from income of fiscal year 2013, the Grant Number: R2556C081).
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The authors declare that they have no conflict of interest.
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Yiemwattana, I., Kaomongkolgit, R. Alpha-mangostin suppresses IL-6 and IL-8 expression in P. gingivalis LPS-stimulated human gingival fibroblasts. Odontology 103, 348–355 (2015). https://doi.org/10.1007/s10266-014-0160-7
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DOI: https://doi.org/10.1007/s10266-014-0160-7