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Loss of chloroplast-localized NAD kinase causes ROS stress in Arabidopsis thaliana

  • Regular Paper – Physiology/Biochemistry/Molecular and Cellular Biology
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Abstract

Chloroplast-localized NAD kinase (NADK2) is responsible for the production of NADP+, which is an electron acceptor in the linear electron flow of photosynthesis. The Arabidopsis T-DNA-inserted mutant of NADK2 (nadk2) showed delayed growth and pale-green leaves under continuous light conditions. Under short-day conditions (8 h light / 16 h dark), the nadk2 mutant showed more severe growth inhibition.The genomic fragment containing the promoter and coding region of NADK2 complemented the phenotypes of nadk2 obtained under continuous light and short-day conditions. The nadk2 mutant produced higher amounts of H2O2 and O2−, which were reduced in the complementary line. Under short-day conditions, the nadk2 mutant accumulated more H2O2 than under continuous light conditions. The accumulation of ascorbate and up-regulation of the PDF1.2 and PR1 genes indicated that the nadk2 mutant is under ROS stress and responding to keep its living activities.

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Data availability

No new datasets were generated or analyzed in this study.

Abbreviations

DAB 3:

3-Diaminobenzidine

H2O2 :

Hydrogen peroxide

NADP:

Nicotinamide adenine dinucleotide phosphate

NAD+ :

Nicotinamide adenine dinucleotide

NBT:

Nitro blue tetrazolium chloride

ROS:

Reactive oxygen species

WT:

Wild type

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Funding

This work was supported by KAKENHI Grant Numbers 19H04715 and 21H05647 to M. K-Y.

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Authors and Affiliations

  1. Graduate School of Science and Engineering, Saitama University, 225 Shimo-Okubo, Sakura-Ku, Saitama-City, Saitama, 338-8570, Japan

    Chaomurilege, Yanhui Zu, Atsuko Miyagi, Toshiki Ishikawa, Masatoshi Yamaguchi & Maki Kawai-Yamada

  2. Graduate School of Faculty of Agriculture, Yamagata University, 1-23, Wakaba-Machi, , Tsuruoka-Shi, Yamagata, 997-8555, Japan

    Atsuko Miyagi

  3. Sustainable Systems Research Laboratory, Biological and Environmental Chemistry Research Division, Central Research Institute of Electric Power Industry (CRIEPI), 1646 Abiko, Chiba, 270-1194, Japan

    Shin-Nosuke Hashida

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  1. Chaomurilege
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  2. Yanhui Zu
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  4. Shin-Nosuke Hashida
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  7. Maki Kawai-Yamada
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Contributions

MKY. and C. designed the study. C., YZ., AM., SNH, and MKY. performed the experiments. C., AM., TI., MY. and MKY. interpreted the data. C. and MKY. wrote the manuscript.

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Correspondence to Maki Kawai-Yamada.

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Chaomurilege, Zu, Y., Miyagi, A. et al. Loss of chloroplast-localized NAD kinase causes ROS stress in Arabidopsis thaliana. J Plant Res 136, 97–106 (2023). https://doi.org/10.1007/s10265-022-01420-w

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  • DOI: https://doi.org/10.1007/s10265-022-01420-w

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