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The ability of P700 oxidation in photosystem I reflects chilling stress tolerance in cucumber

Journal of Plant Research volume 135, pages 681–692 (2022)Cite this article

Abstract

Low temperature inhibits photosynthesis and negatively affects plant growth. Cucumber (Cucumis sativus L.) is a chilling-sensitive plant, and its greenhouse production requires considerable energy during the winter. Therefore, a useful stress marker for selecting chilling-tolerant cucumber cultivars is desirable. In this study, we evaluated chilling-stress damage in different cucumber cultivars by measuring photosynthetic parameters. The majority of cultivars showed decreases in the quantum yield of photosystem (PS) II [Fv/Fm and Y(II)] and the quantity of active PS I (Pm) after chilling stress. In contrast, Y(ND)—the ratio of the oxidized state of PSI reaction center chlorophyll P700 (P700+)—differed among cultivars and was perfectly inversely correlated with Y(NA)—the ratio of the non-photooxidizable P700. It has been known that P700+ accumulates under stress conditions and protects plants to suppress the generation of reactive oxygen species. In fact, cultivars unable to induce Y(ND) after chilling stress showed growth retardation with reductions in chlorophyll content and leaf area. Therefore, Y(ND) can be a useful marker to evaluate chilling-stress tolerance in cucumber.

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Acknowledgements

We would like to thank Mr. Shintaro Matsui, Takii Shubyo, Japan for his helpful suggestions.

Funding

This work was supported by JST, CREST, Japan, Grant Number JPMJCR15O3 and JPMJCR17O2 to Kentaro Ifuku and Chikahiro Miyake.

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Authors and Affiliations

  1. Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan

    Ko Takeuchi, Yufen Che & Takeshi Nakano

  2. Graduate School of Agriculture, Kobe University, Kobe, Hyogo, Japan

    Chikahiro Miyake

  3. Graduate School of Agriculture, Kyoto University, Kitashirakawa oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan

    Kentaro Ifuku

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  1. Ko Takeuchi
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  2. Yufen Che
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  3. Takeshi Nakano
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  4. Chikahiro Miyake
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  5. Kentaro Ifuku
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KI, TN, and CM conceived the project; KT and YC performed the experiments; and KT and KI wrote the manuscript.

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Correspondence to Kentaro Ifuku.

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Takeuchi, K., Che, Y., Nakano, T. et al. The ability of P700 oxidation in photosystem I reflects chilling stress tolerance in cucumber. J Plant Res 135, 681–692 (2022). https://doi.org/10.1007/s10265-022-01404-w

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Keywords

  • Chilling stress
  • Cucumber
  • Photoinhibition
  • Photosynthesis
  • Photosystem I
  • P700 oxidation